A phenanthrene derivative with a pendant methanol amidogen TFCPIP and its two ruthenium(II) complexes [Ru(phen) 2 (TFCPIP)](ClO 4 ) 2 ( 1 ) and [Ru(dmp) 2 (TFCPIP)](ClO 4 ) 2 ( 2 ) (phen = 1,10-phenanthroline, dmp = 2,9-dimethyl-1,10-phenanthroline, TFCPIP = 2-(2,3,5,6-tetrafluoro-4-aminoethanolphenyl)[4,5- f ]-imidazo[1,10]phenanthroline) were synthesized, and the anticancer properties of the two complexes were examined. Both the complexes displayed certain anticancer activities against the selected SGC-7901, BEL-7402, HeLa, A549, MG-63, HepG2, PC-12 and SiHa cancer cells, with the highest cytotoxic activities against SGC-7901. The cell biology experiments for exploring toxicity mechanism with fluorescence imaging technique and flow cytometry demonstrated that the complexes can trigger apoptosis of SGC-7901 cells with an increase in ROS level, a decrease in mitochondrial membrane potential, and effectively inhibit cell invasion and cell growth at G2/M phase, implicating that the complexes induce cellular apoptosis through a ROS-mediated mitochondrial dysfunction pathway.

中文翻译：

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含有悬垂甲醇酰胺基的钌 (II) 复合物通过 ROS 介导的线粒体功能障碍途径诱导 SGC-7901 细胞凋亡

具有悬垂甲醇酰胺基 TFCPIP 的菲衍生物及其两个钌 (II) 配合物 [Ru(phen) 2 (TFCPIP)](ClO 4 ) 2 ( 1 ) 和 [Ru(dmp) 2 (TFCPIP)](ClO 4 ) 2 ( 2 ) (phen = 1,10-菲咯啉，dmp = 2,9-二甲基-1,10-菲咯啉，TFCPIP = 2-(2,3,5,6-四氟-4-氨基乙醇苯基)[4,5 - f ]-咪唑并[1,10]菲咯啉）被合成，并检查了两种复合物的抗癌特性。两种复合物都对选定的 SGC-7901、BEL-7402、HeLa、A549、MG-63、HepG2、PC-12 和 SiHa 癌细胞显示出一定的抗癌活性，对 SGC-7901 的细胞毒活性最高。用荧光成像技术和流式细胞术探索毒性机制的细胞生物学实验表明，随着ROS水平的增加，复合物可以触发SGC-7901细胞的凋亡，