Amorphophallus paeoniifolius known as elephant foot yam is an underutilized tuberous food crop grown primarily in Africa, South Asia, and tropical Pacific islands. Aim of the present work was to isolate, identify and study various physicochemical characteristics of calcium oxalate crystals present in elephant foot yam. The calcium oxalate crystals from elephant foot yam were isolated using freeze thawing followed by aqueous extraction method. The optimum isolation conditions for maximum calcium oxalate yield was found as grinding time of 12.5 min, solid to liquid ratio of 1:2, temperature of 50 °C, grinding speed of 2000 rpm and 3 extraction cycles. Morphological characteristics of isolated calcium oxide crystals were studied using Scanning Electron Microscope and the presence of calcium oxalate crystals in the form of whewellite was confirmed. The purity of isolated calcium oxalate crystals was confirmed using Energy Dispersive x-ray Spectroscopy. The spherical and oval shapes of calcium oxalate crystals were confirmed using Transmission Electron Microscope. X-Ray Diffraction analysis has confirmed the tetragonal phase structure of isolated calcium oxalate crystals with good crystal stability. Calcium oxalate crystals from elephant foot yam were found stable at 175 °C using Thermal Gravimetric and Differential Scanning Calorimetric analysis.

中文翻译：

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morph草生物来源草酸钙晶体的分离与表征

魔芋被称为象脚山药的一种未充分利用的块茎粮食作物，主要生长在非洲，南亚和热带太平洋岛屿。本工作的目的是分离，鉴定和研究象脚山药中存在的草酸钙晶体的各种物理化学特征。冷冻解冻后水萃取法分离大象山药中的草酸钙晶体。草酸钙最大产率的最佳分离条件为研磨时间为12.5分钟，固液比为1：2，温度为50°C，研磨速度为2000 rpm和3个萃取循环。使用扫描电子显微镜研究了分离的氧化钙晶体的形态学特征，并证实了以钙镁铝榴石形式存在的草酸钙晶体的存在。分离的草酸钙晶体的纯度使用能量分散X射线光谱法确认。使用透射电子显微镜确认草酸钙晶体的球形和椭圆形。X射线衍射分析已证实分离的草酸钙晶体的四方相结构具有良好的晶体稳定性。使用热重分析和差示扫描量热法分析，发现象脚山药中的草酸钙晶体在175°C时稳定。