Abstract
Amorphophallus paeoniifolius known as elephant foot yam is an underutilized tuberous food crop grown primarily in Africa, South Asia, and tropical Pacific islands. Aim of the present work was to isolate, identify and study various physicochemical characteristics of calcium oxalate crystals present in elephant foot yam. The calcium oxalate crystals from elephant foot yam were isolated using freeze thawing followed by aqueous extraction method. The optimum isolation conditions for maximum calcium oxalate yield was found as grinding time of 12.5 min, solid to liquid ratio of 1:2, temperature of 50 °C, grinding speed of 2000 rpm and 3 extraction cycles. Morphological characteristics of isolated calcium oxide crystals were studied using Scanning Electron Microscope and the presence of calcium oxalate crystals in the form of whewellite was confirmed. The purity of isolated calcium oxalate crystals was confirmed using Energy Dispersive x-ray Spectroscopy. The spherical and oval shapes of calcium oxalate crystals were confirmed using Transmission Electron Microscope. X-Ray Diffraction analysis has confirmed the tetragonal phase structure of isolated calcium oxalate crystals with good crystal stability. Calcium oxalate crystals from elephant foot yam were found stable at 175 °C using Thermal Gravimetric and Differential Scanning Calorimetric analysis.
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Abbreviations
- ADA:
-
American Dietetic Association
- DLS:
-
Dynamic light scattering
- TGA:
-
Thermogravimetric analysis
- TG-DSC:
-
Thermogravimetric-differential scanning calorimeter
References
ADA (American Dietetic Association) (2005) Urolithiasis/urinary stones. In ADA nutrition care, Manual No. 275. American Dietetic Association, Chicago, USA
Al-Wahsh IA, Wu Y, Liebman M (2012) A Comparison of two extraction methods for food oxalate assessment. J Food Res 1:233–239
Chattopadhyay A, Saha P, Pal S, Bhattacharya A, Sen H (2009) Quantitative and qualitative aspects of elephant foot yam. Int J Veg Sci 16:73–84
da Costa LM, Tronto J, Constantino VRL, Fonseca MKA, Oliveira AP, da Costa MR (2009) Extraction and concentration of biogenic calcium oxalate from plant leaves. Rev Bras Ciênc Solo 33:729–733
Du Thanh H, Phan VuH, Van Vu H, Le Duc N, Le Minh T, Savage G (2017) Oxalate content of taro leaves grown in central Vietnam. Foods 6:2
Englberger L, Aalbersberg W, Ravi P, Bonnin M, Marks GC, Fitzgerald MH, Elymore J (2003) Further analysis on Micronesia banana, taro, breadfruit and other foods for provitamin-A carotenoids and minerals. J Food Compost Anal 16:216–236
Guil-Guerrero JL (2014) The safety of wild edible plants: fuller discussion may be needed. J Food Compost Anal 35:18–20
Holmes RP, Goodman HO, Assimos DG (2001) Contribution of dietary oxalate to urinary oxalate excretion. Kidney Int 59:270–276
Kumar A, Patel AA, Gupta VK (2017) Reduction in oxalate, acridity, phenolic content and antioxidant activity of Amorphophallus paeoniifolius var. Gajendra upon cooking. Int Food Res J 24:1614–1620
Kumoro AC, Budiyati CS, Retnowati DS (2014) Calcium oxalate reduction during soaking of giant taro (Alocasia macrorrhiza (L.) Schott) corm chips in sodium bicarbonate solution. Int Food Res J 21:1583–1588
Lewu MN, Adebola PO, Afolayan AJ (2010) Effect of cooking on the mineral contents and anti-nutritional factors in seven accessions of Colocasia esculenta (L.) Schott growing in South Africa. J Food Compost Anal 23:389–393
Libert B, Franceschi VR (1987) Oxalate in crop plants. J Agric Food Chem 35:926–937
Mukhopadhyay SK, Sen H (1999) Effect of Azotobacter on corm yield of elephant foot yam. J Root Crops 25:159–162
Parvathi, Subbulakshmi B (2016) Development of value added food products from tropical tubers. J Crop Weed 12(1):69–75
Ramakumar P (2011) The thickening effect of elephant foot yam in milk systems. M.Tech thesis, National Dairy Research Institute, Karnal, India
Sahoo B, Nedunchezhiyan M, Acharyya P (2016) Incidence of collar rot in elephant foot yam [Amorphophallus paeoniifolius, (Dennst.) Nicolson] as influenced by varied nutrient regimes in East and South-eastern coastal plain zone of Odisha. J Crop Weed 12(3):160–162
Santosa E, Lian CL, Sugiyama N, Misra RS, Boonkorkaew P, Thanomchit K (2017) Population structure of elephant foot yams (Amorphophallus paeoniifolius (Dennst.) Nicolson) in Asia. PLoS ONE 12(6):e0180000. https://doi.org/10.1371/journal.pone.0180000
Subashini S, Sathish Kumar K (2017) Physicochemical characteristics of calcium oxalate crystals in Spinacia oleracea L. Indian J Biochem Biophy 54:156–163
Williams HE, Wandzilak TR (1989) Oxalate synthesis, transport and the hyperoxaluric syndromes. J Urol 141:742–747
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Subashini, S., Sathish Kumar, K. Isolation and characterization of biogenic calcium oxalate crystals from Amorphophallus paeoniifolius. J. Plant Biochem. Biotechnol. 28, 382–388 (2019). https://doi.org/10.1007/s13562-019-00493-4
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DOI: https://doi.org/10.1007/s13562-019-00493-4