The biosynthesis of selenium nanoparticles is performed with the traditionally used medicinal seed of Mucuna pruriens and the synthesis rate of a product is stabilized when the experimental conditions are well operated, to serve this purpose optimization techniques like response surface methodology has been followed. The technique is employed for analysing the average size of the selenium nanoparticles as the response. The variables included are precursor concentration, seed extract concentration and time taken for the synthesis, also the interactive conditions against the size were evaluated, on the basis of quadratic equation constructed with high R² (coefficient of determination) value of 98%. The responses were collected from DLS and the nanoparticles were further characterized using SEM, TEM, AFM, XRD, and FTIR. The size of the optimized nanoparticles produced was nearly 100–120 nm validated by the software and also from various characterization tools. The optimized SeNPs were subjected to antioxidants through DPPH assay, in which the IC₅₀ value was 60 µg/mL. The cell viability was also evaluated, the calculated IC₅₀ was 40 µg/mL at 48 h, and for 24 h the IC₅₀ was 80 µg/mL. The cost-effective and environmental friendly selenium nanoparticles can be utilized further for future biomedical applications.

中文翻译：

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生物合成硒纳米粒子对A549肺癌细胞株的细胞毒性分析

硒纳米颗粒的生物合成是通过传统使用的of豆（Mucuna pruriens）的药用种子进行的，并且在实验条件良好的情况下，产品的合成速率稳定，以达到该目的，遵循了响应面方法之类的优化技术。该技术用于分析硒纳米颗粒的平均尺寸作为响应。包括的变量是前体浓度，种子提取物浓度和合成所需的时间，还基于高R ²构造的二次方程评估了与尺寸有关的相互作用条件。（测定系数）值为98％。从DLS收集响应，并使用SEM，TEM，AFM，XRD和FTIR对纳米颗粒进行进一步表征。通过软件以及各种表征工具验证的最优化纳米粒子的大小约为100–120 nm。通过DPPH分析法对优化的SeNPs进行抗氧化剂处理，IC ₅₀值为60 µg / mL。还评估了细胞活力，在48 h时计算出的IC ₅₀为40 µg / mL，在24 h中IC ₅₀为80 µg / mL。具有成本效益且环保的硒纳米颗粒可进一步用于未来的生物医学应用。