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Cytotoxicity Analysis of Biosynthesized Selenium Nanoparticles Towards A549 Lung Cancer Cell Line

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Abstract

The biosynthesis of selenium nanoparticles is performed with the traditionally used medicinal seed of Mucuna pruriens and the synthesis rate of a product is stabilized when the experimental conditions are well operated, to serve this purpose optimization techniques like response surface methodology has been followed. The technique is employed for analysing the average size of the selenium nanoparticles as the response. The variables included are precursor concentration, seed extract concentration and time taken for the synthesis, also the interactive conditions against the size were evaluated, on the basis of quadratic equation constructed with high R2 (coefficient of determination) value of 98%. The responses were collected from DLS and the nanoparticles were further characterized using SEM, TEM, AFM, XRD, and FTIR. The size of the optimized nanoparticles produced was nearly 100–120 nm validated by the software and also from various characterization tools. The optimized SeNPs were subjected to antioxidants through DPPH assay, in which the IC50 value was 60 µg/mL. The cell viability was also evaluated, the calculated IC50 was 40 µg/mL at 48 h, and for 24 h the IC50 was 80 µg/mL. The cost-effective and environmental friendly selenium nanoparticles can be utilized further for future biomedical applications.

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Acknowledgements

The authors did not face any disagreement while doing the work and would like to thank the Vellore Institute of Technology for encouragement, seed fund and support bestowed upon us.

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  1. School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632014, India

    Soumya Menon & VenkatKumar Shanmugam

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Correspondence to VenkatKumar Shanmugam.

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Menon, S., Shanmugam, V. Cytotoxicity Analysis of Biosynthesized Selenium Nanoparticles Towards A549 Lung Cancer Cell Line. J Inorg Organomet Polym 30, 1852–1864 (2020). https://doi.org/10.1007/s10904-019-01409-4

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