This study describes the biocontrol potential of rhizobacteria against a range of fungal phytopathogens. Out of 227 bacteria isolated from the rhizosphere of maize, rice, wheat, potato, sunflower and soybean crops cultivated in different agro-ecological regions of Pakistan, 48 exhibited >60 % antifungal activity against Fusarium oxysporum, Fusarium moniliforme, Rhizoctonia solani, Colletotrichum gloeosporioides, Colletotrichum falcatum, Aspergillus niger, and Aspergillus flavus. The rhizobacteria inhibiting >65 % pathogen growth were selected for detailed molecular and in planta studies most of which were identified as Pseudomonas and Bacillus species based on 16S rRNA gene sequence analysis. Antifungal metabolites produced by these rhizobacteria analyzed through LCMS were identified as antibiotics (iturin, surfactins, fengycin, DAPG, Phenazine, etc.), cell wall degrading enzymes (protease, chitinase, and cellulase), plant growth promotion enzymes and hormones (indole-3-acetic acid, ACC-deaminase, phosphates, nitrogen fixation), N-acyl-homoserine lactones and siderophores. The growth room experiment validated the potential of these bacteria as biofertilizer and biopesticide agents. Of all, P. aeruginosa strain FB2 and B. subtilis strain RMB5 showed significantly higher potential as antagonistic plant-beneficial bacteria effective against a range of fungal phytopathogens. Both these bacteria can be used to develop a dual-purpose bacterial inoculum as biopesticide and biofertilizer. Rest of the antagonistic PGPR may be exploited for disease control in less-infested soils.

这项研究描述了根瘤菌对一系列真菌性植物病原体的生物防治潜力。从巴基斯坦不同农业生态区种植的玉米，水稻，小麦，马铃薯，向日葵和大豆作物的根际分离出的227种细菌中，有48种对尖孢镰刀菌，尖孢镰刀菌，根瘤菌，茄形炭疽菌具有超过60％的抗真菌活性。，炭疽菌，黑曲霉和黄曲霉。选择抑制根瘤菌> 65％病原体生长的根瘤菌进行详细的分子研究，在植物研究中，根据16S rRNA，大多数被鉴定为假单胞菌属和芽孢杆菌属。基因序列分析。这些通过LCMS分析的根瘤菌产生的抗真菌代谢物被鉴定为抗生素（维他命，表面活性素，丰霉素，DAPG，苯那嗪等），细胞壁降解酶（蛋白酶，几丁质酶和纤维素酶），植物生长促进酶和激素（吲哚- 3-乙酸，ACC脱氨酶，磷酸盐，固氮），N-酰基高丝氨酸内酯和铁载体。生长室实验验证了这些细菌作为生物肥料和生物农药的潜力。在所有铜绿假单胞菌菌株FB2和枯草芽孢杆菌中菌株RMB5表现出显着较高的潜力，可作为对多种真菌性植物病原体有效的拮抗植物有益细菌。这两种细菌均可用于开发生物杀虫剂和生物肥料的双重用途细菌接种物。其余的PGPR拮抗药可用于少感染土壤中的疾病控制。