MicroRNAs (miRNAs) regulate a wide range of cellular processes and functions. Blood mononuclear cells (BMNCs) participate in the immune response, inflammatory reaction and angiogenesis. In 2010, a total of 157 miRNAs were quantified by RT-qPCR and a miRNA signature was determined for human peripheral BMNCs. With the advent of technologies such as RNA sequencing, many new miRNAs have been identified. This study was designed to provide an up-to-date miRNA signature for human BMNCs. Peripheral BMNCs were isolated by Ficoll density gradient centrifugation. Using the qPCR array assay, we identified 108 highly expressed miRNAs (Ct value < 30) in human BMNCs. Further validation of the array results by quantifying select miRNAs with RT-qPCR revealed a strong correlation between Ct values derived from array analysis and RT-qPCR, suggesting the array results presented in this study are accurate and reliable. Of note, the function of the majority of the highly expressed miRNAs we have identified has not yet been studied. Our findings may help direct further studies of the regulatory roles of miRNAs in BMNC function.

中文翻译：

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人血单核细胞的 MicroRNA 特征。

MicroRNAs (miRNAs) 调节广泛的细胞过程和功能。血液单核细胞（BMNCs）参与免疫反应、炎症反应和血管生成。2010 年，通过 RT-qPCR 对总共 157 种 miRNA 进行了定量，并确定了人类外周 BMNC 的 miRNA 特征。随着 RNA 测序等技术的出现，已经鉴定出许多新的 miRNA。本研究旨在为人类 BMNCs 提供最新的 miRNA 特征。通过Ficoll密度梯度离心分离外周BMNC。使用 qPCR 阵列分析，我们在人类 BMNC 中鉴定了 108 个高表达 miRNA（Ct 值 < 30）。通过使用 RT-qPCR 量化选择的 miRNA 进一步验证阵列结果揭示了来自阵列分析和 RT-qPCR 的 Ct 值之间的强相关性，表明本研究中提出的阵列结果是准确可靠的。值得注意的是，我们发现的大多数高表达 miRNA 的功能尚未得到研究。我们的研究结果可能有助于指导进一步研究 miRNA 在 BMNC 功能中的调节作用。