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MicroRNA signature of human blood mononuclear cells

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Abstract

MicroRNAs (miRNAs) regulate a wide range of cellular processes and functions. Blood mononuclear cells (BMNCs) participate in the immune response, inflammatory reaction and angiogenesis. In 2010, a total of 157 miRNAs were quantified by RT-qPCR and a miRNA signature was determined for human peripheral BMNCs. With the advent of technologies such as RNA sequencing, many new miRNAs have been identified. This study was designed to provide an up-to-date miRNA signature for human BMNCs. Peripheral BMNCs were isolated by Ficoll density gradient centrifugation. Using the qPCR array assay, we identified 108 highly expressed miRNAs (Ct value < 30) in human BMNCs. Further validation of the array results by quantifying select miRNAs with RT-qPCR revealed a strong correlation between Ct values derived from array analysis and RT-qPCR, suggesting the array results presented in this study are accurate and reliable. Of note, the function of the majority of the highly expressed miRNAs we have identified has not yet been studied. Our findings may help direct further studies of the regulatory roles of miRNAs in BMNC function.

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Author notes

  1. Qiuwang Zhang and Anthony Cannavicci contributed equally to this work.

Authors and Affiliations

  1. Division of Cardiology, Keenan Research Center for Biomedical Science, St. Michael’s Hospital, University of Toronto, Toronto, ON, Canada

    Qiuwang Zhang, Anthony Cannavicci, Si-Cheng Dai & Michael J. B. Kutryk

  2. Institute of Medical Science, University of Toronto, Toronto, ON, Canada

    Anthony Cannavicci & Michael J. B. Kutryk

  3. Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiaotong University, Shanghai, China

    Chenxi Wang

  4. St. Michael’s Hospital, Room 7084, Bond Wing, 30 Bond Street, Toronto, ON, M5B 1W8, Canada

    Michael J. B. Kutryk

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  1. Qiuwang Zhang
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  2. Anthony Cannavicci
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  3. Si-Cheng Dai
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  4. Chenxi Wang
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  5. Michael J. B. Kutryk
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Correspondence to Michael J. B. Kutryk.

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Zhang, Q., Cannavicci, A., Dai, SC. et al. MicroRNA signature of human blood mononuclear cells. Mol Cell Biochem 462, 167–172 (2019). https://doi.org/10.1007/s11010-019-03619-3

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  • DOI: https://doi.org/10.1007/s11010-019-03619-3

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