Microencapsulation of partially purified microbial transglutaminase (mTG) was investigated using ultrasonic spray-freeze drying (USFD), and the optimum coating materials (gum arabic, maltodextrin, inulin) ratio and the process parameters (flow rate and nozzle frequency) were determined using a D-optimal combined design. Also, the microencapsulated samples by USFD were compared with microencapsulated samples by conventional freeze drying (CFD) and conventional spray drying (CSD) in terms of microencapsulation efficiency, enzyme stability at extreme pH and high temperature conditions, and the presence of metal ions, physical (moisture content, particle morphology, particle and pore size, surface area, pore volume distribution, density and flow properties, caking degree, color), and reconstitution (wettability and solubility) properties. As a result, the optimum coating materials composition was determined as 60% gum arabic and 40% inulin, and process conditions were found to be flow rate of 6.83 ml/min and nozzle frequency of 48 kHz applying desirability function method. Microcapsules with smaller particle size, pore volume, and porosity, with lower moisture content and good reconstitution characteristics, were obtained by USFD with a maximum microencapsulation efficiency of ~ 97%.

使用超声喷雾冷冻干燥（USFD）研究了部分纯化的微生物转谷氨酰胺酶（mTG）的微囊化，并使用D最佳组合设计。此外，将USFD的微囊化样品与常规冷冻干燥（CFD）和常规喷雾干燥（CSD）的微囊化样品的微囊化效率，在极端pH和高温条件下的酶稳定性以及金属离子的存在，物理稳定性方面进行了比较。 （水分含量，颗粒形态，颗粒和孔径，表面积，孔体积分布，密度和流动性，结块度，颜色）和重构（润湿性和溶解度）特性。因此，确定了最佳的涂料组成为阿拉伯胶60％和菊粉40％，采用理想功能法确定的工艺条件为流速为6.83 ml / min，喷嘴频率为48 kHz。通过USFD获得了具有较小的粒径，孔体积和孔隙率，较低的水分含量和良好的重构特性的微囊，其最大微囊化效率约为97％。