Surface plasmon resonance microscopy and imaging are optical methods that enable observation and quantification of interactions of nano- and microscale objects near a metal surface in a temporally and spatially resolved manner. This review describes the principles of surface plasmon resonance microscopy and imaging and discusses recent advances in these methods, in particular, in optical platforms and functional coatings. In addition, the biological applications of these methods are reviewed. These include the detection of a broad variety of analytes (nucleic acids, proteins, bacteria), the investigation of biological systems (bacteria and cells), and biomolecular interactions (drug-receptor, protein-protein, protein-DNA, protein-cell).

中文翻译：

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表面等离子体共振成像和显微镜及其生物学应用的进展。

表面等离子体共振显微镜和成像是光学方法，能够以时间和空间分辨的方式观察和量化金属表面附近的纳米级和微米级物体的相互作用。这篇综述描述了表面等离子体共振显微镜和成像的原理，并讨论了这些方法的最新进展，特别是在光学平台和功能涂层方面。另外，综述了这些方法的生物学应用。这些包括检测各种分析物（核酸，蛋白质，细菌），研究生物系统（细菌和细胞）以及生物分子相互作用（药物受体，蛋白质-蛋白质，蛋白质-DNA，蛋白质-细胞） 。