Methicillin-resistant Staphylococcus aureus (MRSA) transmission in livestock, community, and healthcare settings poses a significant public health concern both locally and globally. This study aimed to investigate the occurrence, molecular detection, and antibiogram of the MRSA strain in fresh beef, contact surfaces, and butchers’ knives from the four major abattoirs (Karu, Gwagwalada, Deidei, and Kubwa) located in the Federal Capital Territory, Nigeria. A multi-stage sampling technique was used to collect 400 swab samples from butchers’ knives (132), fresh beef (136), and contact surfaces (132). Presumptive colonies on mannitol salt agar were subjected to culture, isolation, and biotyping. The antibiogram was carried out via a Kirby-Bauer disk containing eight antibiotics. MRSA was phenotypically confirmed by oxacillin-resistant screening agar base (ORSAB) and genotypically by PCR to detect the presence of the mecA gene. Out of the 400 samples, 47.24% of fresh beef, 37% of contact surfaces, and 64.33% of butchers’ knife swabs were Staphylococcus aureus positive. Thirty-two Staphylococcus aureus-positive isolates were confirmed to be MRSA, 50% fresh beef, 28.12% contact surfaces, and 21.87% butcher’s knife swabs. MRSA isolates displayed multidrug-resistant traits, with a high resistance of 90.62% against cloxacillin, and a highest susceptibility of 100% to co-trimaxole. The antibiogram showed MRSA strains to be multidrug resistant. Molecular characterisation of the MRSA detected the presence of the mecA gene at a band size of 163 bp in all isolates. Strict hygiene of butchers, and working equipment in meat processing and marketing should be of top priority.

耐甲氧西林金黄色葡萄球菌(MRSA) 在牲畜、社区和医疗机构中的传播给当地和全球带来了重大的公共卫生问题。本研究旨在调查位于联邦首都地区四个主要屠宰场（卡鲁、格瓦格瓦拉达、代德伊和库布瓦）的新鲜牛肉、接触面和屠宰刀中 MRSA 菌株的出现情况、分子检测和抗菌谱。尼日利亚。采用多阶段采样技术从屠刀 (132)、新鲜牛肉 (136) 和接触表面 (132) 收集了 400 个拭子样本。对甘露醇盐琼脂上的假定菌落进行培养、分离和生物分型。通过含有八种抗生素的 Kirby-Bauer 圆盘进行抗菌谱分析。 MRSA 通过苯唑西林抗性筛选琼脂碱 (ORSAB) 进行表型证实，并通过 PCR 检测mecA基因的存在进行基因型证实。在400个样本中，47.24%的新鲜牛肉、37%的接触面和64.33%的屠宰刀拭子呈金黄色葡萄球菌阳性。 32 株金黄色葡萄球菌阳性分离株被确认为 MRSA，其中 50% 为新鲜牛肉，28.12% 为接触面，21.87% 为屠宰刀拭子。 MRSA分离株表现出多重耐药性，对氯唑西林的耐药率高达90.62%，对复方曲马索的敏感性最高为100%。抗菌谱显示 MRSA 菌株具有多重耐药性。 MRSA 的分子表征在所有分离株中检测到mecA基因的存在，条带大小为 163 bp。严格屠宰者的卫生以及肉类加工和销售中的工作设备应是重中之重。