The purpose is to assess the efficacy of rose bengal photodynamic antimicrobial therapy (PDAT) using different irradiation energy levels and photosensitizer concentrations for the inhibition of fungal keratitis isolates. Seven different fungi (Aspergillus fumigatus, Candida albicans, Curvularia lunata, Fusarium keratoplasticum, Fusarium solani, Paecilomyces variotii, and Pseudallescheria boydii) were isolated from patients with confirmed infectious keratitis. Experiments were performed in triplicate with suspensions of each fungus exposed to different PDAT parameters including a control, green light exposure of 5.4 J/cm², 2.7 J/cm² (continuous and pulsed), and 1.8 J/cm² and rose bengal concentrations of 0.1%, 0.05%, and 0.01%. Plates were photographed 72 h after experimentation, and analysis was performed to assess fungal growth inhibition. PDAT using 5.4 J/cm² of irradiation and 0.1% rose bengal completely inhibited growth of five of the seven fungal species. Candida albicans and Fusarium keratoplasticum were the most susceptible organisms, with growth inhibited with the lowest fluence and minimum rose bengal concentration. Fusarium solani, Pseudallescheria boydii, and Paecilomyces variotii were inhibited by lower light exposures and photosensitizer concentrations. Aspergillus fumigatus and Curvularia lunata were not inhibited by any PDAT parameters tested. Continuous and pulsed irradiation using 2.7 J/cm² produced similar results. Rose bengal PDAT successfully inhibits the in vitro growth of five fungi known to cause infectious keratitis. Differences in growth inhibition of the various fungi to multiple PDAT parameters suggest that susceptibilities to PDAT are unique among fungal species. These findings support modifying PDAT parameters based on the infectious etiology.

目的是评估使用不同照射能量水平和光敏剂浓度的孟加拉玫瑰光动力抗菌疗法（PDAT）抑制真菌性角膜炎分离株的功效。从确诊的感染性角膜炎患者中分离出七种不同的真菌（烟曲霉、白色念珠菌、新月弯孢菌、角膜塑性镰刀菌、茄病镰刀菌、变异拟青霉和博伊拟青霉）。实验一式三份进行，每种真菌的悬浮液暴露于不同的 PDAT 参数，包括对照、绿光暴露 5.4 J/cm ²、2.7 J/cm ²（连续和脉冲）和 1.8 J/cm ²以及玫瑰红浓度0.1%、0.05% 和 0.01%。实验后72小时对板进行拍照，并进行分析以评估真菌生长抑制。使用5.4 J/cm ²辐射和0.1%孟加拉玫瑰的PDAT完全抑制了七种真菌中的五种的生长。白色念珠菌和角化镰孢菌是最敏感的微生物，其生长受到最低注量和最低孟加拉玫瑰浓度的抑制。较低的光照和光敏剂浓度可抑制茄病镰刀菌、博氏拟青霉和拟青霉。任何测试的 PDAT 参数均不会抑制烟曲霉和新月弯孢霉。使用2.7 J/cm ²的连续和脉冲照射产生类似的结果。孟加拉玫瑰 PDAT 成功抑制五种已知引起感染性角膜炎的真菌的体外生长。各种真菌对多个 PDAT 参数的生长抑制差异表明，真菌物种对 PDAT 的敏感性是独特的。这些发现支持根据感染病因修改 PDAT 参数。