Protein stability is important for biological function, but little is known about in-cell stability. In the New Delhi metallo-β-lactamase NDM-1, enhancement of zinc binding or amino acid substitutions at the C terminus increase in-cell kinetic stability and prevent proteolysis. These findings link NDM-1-mediated resistance with its in-cell stability and physiology.

中文翻译：

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细胞内蛋白质稳定性促进金属-β-内酰胺酶的抗菌耐药性

蛋白质稳定性对于生物功能很重要，但人们对细胞内稳定性知之甚少。在新德里金属-β-内酰胺酶 NDM-1 中，C 末端锌结合的增强或氨基酸取代可增加细胞内动力学稳定性并防止蛋白水解。这些发现将 NDM-1 介导的耐药性与其细胞内稳定性和生理学联系起来。