Protein stability is important for biological function, but little is known about in-cell stability. In the New Delhi metallo-β-lactamase NDM-1, enhancement of zinc binding or amino acid substitutions at the C terminus increase in-cell kinetic stability and prevent proteolysis. These findings link NDM-1-mediated resistance with its in-cell stability and physiology.
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References
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This is a summary of: González, L. J. et al. In-cell kinetic stability is an essential trait in metallo-β-lactamase evolution. Nat. Chem. Biol. https://doi.org/10.1038/s41589-023-01319-0 (2023).
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In-cell protein stability promotes antimicrobial resistance of metallo-β-lactamases. Nat Chem Biol 19, 1050–1051 (2023). https://doi.org/10.1038/s41589-023-01322-5
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DOI: https://doi.org/10.1038/s41589-023-01322-5
