The present study evaluated the hybrid enzymatic hydrolysis and fermentation of Prosopis juliflora pods (Pj pods) for the enhanced biohydrogen production from the activated anaerobic sludge through the waste to energy approach (WtE). The pretreatment effect of sulfuric acid, citric acid, sodium hydroxide, and ammonia has been studied on the Pj pods to make cellulose more accessible for enzyme hydrolysis. The sulfuric acid pretreatment obtained the maximum glucose, 193.19 mg/g BM. The cellulolytic enzyme cocktail comprising cellulase, 80 IU/g, and xylanase, 234.58 IU/g from the newly isolated Trichoderma harzianum BPGF1, is used to hydrolyze the sulfuric acid-treated Pj pods. After the separate enzymatic hydrolysis, the acid-treated Pj pods produced 342.51 mg/g BM glucose. The hybrid enzymatic hydrolysis and fermentation of Pj pods with activated anaerobic sludge was optimized using the central composite design (CCD). The microbial consortium in the anaerobic sludge was identified by next-generation sequencing (NGS) through metagenomic analysis, and the hydrogen was analyzed by gas chromatography. The maximum biohydrogen yield of 1552.25 ± 53.61 mM/L was achieved in the medium containing Pj pods, 13.6% w/v, vitamin solutions, 1.44% v/v, cellulase, 62.74 IU/g BM, and inoculum, 8.67% v/v at an initial pH of 6.49 under anaerobic conditions.

本研究评估了牧豆荚 (Pj 荚) 的混合酶水解和发酵，以通过废物转化为能源的方法 (WtE) 从活化的厌氧污泥中增强生物氢的生产。研究了硫酸、柠檬酸、氢氧化钠和氨对 Pj 豆荚的预处理效果，以使纤维素更容易被酶水解。硫酸预处理获得最大葡萄糖，193.19 mg/g BM。纤维素分解酶混合物包含来自新分离的哈茨木霉的纤维素酶 80 IU/g 和木聚糖酶 234.58 IU/gBPGF1，用于水解硫酸处理的 Pj 豆荚。在单独的酶水解后，酸处理的 Pj 豆荚产生 342.51 mg/g BM 葡萄糖。使用中心复合设计 (CCD) 优化了 Pj 豆荚与活性厌氧污泥的混合酶水解和发酵。通过宏基因组分析，通过二代测序（NGS）鉴定厌氧污泥中的微生物群落，并通过气相色谱分析氢气。在含有 Pj 豆荚（13.6% w/v）、维生素溶液（1.44% v/v）、纤维素酶（62.74 IU/g BM）和接种物（8.67% v/ v 在厌氧条件下初始 pH 值为 6.49。