A label-free electrochemical biosensor has been developed using zinc oxide nanoflowers (ZnONFs) for the detection of uric acid concentration in human blood serum. ZnONFs have been synthesized by a hydrothermal process and characterized with several techniques such as ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR) studies, X-ray diffraction (XRD) study, Raman spectroscopy, scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HR-TEM) and electrochemical analyzer to confirm the formation of nanoflowers and fabrication of electrode and bio-electrodes for uric acid detection. Zinc Oxide nanoflowers have been deposited onto indium–tin oxide (ITO) substrate through electrophoretic deposition technique, and the biosensor has been fabricated by immobilizing urate oxidase (UOx) enzyme onto ZnONFs/ITO electrode surfaces. Further, electrochemical studies have been performed with immobilized UOx/ZnONFs/ITO bio-electrode as a function of uric acid concentrations. It has been found that the fabricated uric acid biosensor shows a high sensitivity (10.38 μA/mM/cm²) and a limit of detection of 0.13 mM in the range of 0.005 to 1.0 mM. This study demonstrates the potential use of ZnONFs for the construction of sensitive biosensors for uric acid detection.

使用氧化锌纳米花（ZnONFs）开发了一种无标记的电化学生物传感器，用于检测人血清中的尿酸浓度。ZnONFs 已通过水热法合成，并通过多种技术进行表征，例如紫外-可见 (UV-Vis) 光谱、傅里叶变换红外光谱 (FTIR) 研究、X 射线衍射 (XRD) 研究、拉曼光谱、扫描电子显微镜 ( SEM）和高分辨率透射电子显微镜（HR-TEM）和电化学分析仪，以确认纳米花的形成以及用于尿酸检测的电极和生物电极的制造。氧化锌纳米花已通过电泳沉积技术沉积在氧化铟锡 (ITO) 基板上，该生物传感器是通过将尿酸氧化酶 (UOx) 酶固定在 ZnONFs/ITO 电极表面上而制成的。此外，已使用固定的 UOx/ZnONFs/ITO 生物电极进行电化学研究，作为尿酸浓度的函数。已经发现，制造的尿酸生物传感器显示出高灵敏度（10.38 μA/mM/cm² ) 和 0.13 mM 的检测限在 0.005 到 1.0 mM 的范围内。这项研究证明了 ZnONF 在构建用于尿酸检测的敏感生物传感器中的潜在用途。