The aim of this study is to investigate the role of CDGSH iron-sulfur domain 2 (CISD2) in colorectal cancer (CRC). The purpose of this study was to investigate the role of CDGSH iron-sulfur domain 2 (CISD2) in colorectal cancer (CRC) progression. The expression of CISD2 in CRC cell lines was measured by western blotting. Functional assays including MTT assays and colony formation assays were performed to explore the role of CISD2 in regulating tumor growth. Flow cytometry analysis was used to examine the percentage of apoptotic CRC cells. Expression of apoptosis-related gene, autophagy-related markers, and the protein included in Wnt/β-Catenin signaling was also determined by western blotting. The in vivo role of CISD2 was also examined in a xenograft model. CISD2 expression was significantly increased in CRC cells. CISD2 promoted the CRC cell proliferation and inhibited the apoptosis and autophagy of CRC cells. Moreover, knockdown of CISD2 inhibited the activation of Wnt/β-Catenin-signaling pathway. Knockdown of CISD2 inhibited the tumor growth in nude mice. CISD2 promoted colorectal cancer development by inhibiting CRC cell apoptosis and autophagy depending on activating Wnt/β-Catenin-signaling pathway.

本研究的目的是研究 CDGSH 铁硫结构域 2 (CISD2) 在结直肠癌 (CRC) 中的作用。本研究的目的是研究 CDGSH 铁硫结构域 2 (CISD2) 在结直肠癌 (CRC) 进展中的作用。通过蛋白质印迹测量 CRC 细胞系中 CISD2 的表达。进行了包括 MTT 测定和集落形成测定在内的功能测定，以探索 CISD2 在调节肿瘤生长中的作用。流式细胞术分析用于检查凋亡的 CRC 细胞的百分比。细胞凋亡相关基因、自噬相关标记物和 Wnt/β-Catenin 信号中包含的蛋白质的表达也通过蛋白质印迹法测定。还在异种移植模型中检查了 CISD2 的体内作用。CRC 细胞中的 CISD2 表达显着增加。CISD2促进CRC细胞增殖，抑制CRC细胞凋亡和自噬。此外，CISD2 的敲低抑制了 Wnt/β-Catenin 信号通路的激活。CISD2的敲低抑制了裸鼠的肿瘤生长。CISD2 通过激活 Wnt/β-Catenin 信号通路抑制 CRC 细胞凋亡和自噬来促进结直肠癌的发展。