Abstract
The aim of this study is to investigate the role of CDGSH iron-sulfur domain 2 (CISD2) in colorectal cancer (CRC). The purpose of this study was to investigate the role of CDGSH iron-sulfur domain 2 (CISD2) in colorectal cancer (CRC) progression. The expression of CISD2 in CRC cell lines was measured by western blotting. Functional assays including MTT assays and colony formation assays were performed to explore the role of CISD2 in regulating tumor growth. Flow cytometry analysis was used to examine the percentage of apoptotic CRC cells. Expression of apoptosis-related gene, autophagy-related markers, and the protein included in Wnt/β-Catenin signaling was also determined by western blotting. The in vivo role of CISD2 was also examined in a xenograft model. CISD2 expression was significantly increased in CRC cells. CISD2 promoted the CRC cell proliferation and inhibited the apoptosis and autophagy of CRC cells. Moreover, knockdown of CISD2 inhibited the activation of Wnt/β-Catenin-signaling pathway. Knockdown of CISD2 inhibited the tumor growth in nude mice. CISD2 promoted colorectal cancer development by inhibiting CRC cell apoptosis and autophagy depending on activating Wnt/β-Catenin-signaling pathway.
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This work was supported by the 2014 Medical Clinical Science and Technology Development Fund of Jiangsu University (Grant No. JLY201440087).
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JW and JH designed the study, supervised the data collection, MW and HY analyzed the data, interpreted the data, YX, XZ, MD, WC, BQ, and LZ prepare the manuscript for publication and reviewed the draft of the manuscript. All authors have read and approved the manuscript.
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All animal experiments were approved by the Ethics Committee of Gaochun People’s Hospital. For the use of animals and conducted in accordance with the National Institutes of Health Laboratory Animal Care and Use Guidelines.
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Wang, J., Hu, J., Wang, M. et al. CISD2 Promotes Proliferation of Colorectal Cancer Cells by Inhibiting Autophagy in a Wnt/β-Catenin-Signaling-Dependent Pathway. Biochem Genet 61, 615–627 (2023). https://doi.org/10.1007/s10528-022-10267-8
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DOI: https://doi.org/10.1007/s10528-022-10267-8