Skeletal Radiology ( IF 1.9 ) Pub Date : 2022-08-18 , DOI: 10.1007/s00256-022-04149-8 Gloria Vega 1 , Germán Ricaurte 2 , Mauricio Estrada-Castrillón 3, 4 , Harmen Reyngoudt 5 , Oscar M Cardona 6 , Jaime A Gallo-Villegas 4 , Raul Narvaez-Sanchez 1 , Juan C Calderón 1
Objective
To standardize a method for 1H MRS intramuscular absolute quantification of carnosine in the thigh, using a surface coil and water as internal reference.
Materials and methods
Carnosine spectra were acquired in phantoms (5, 10, and 15 mM) as well as in the right gastrocnemius medialis (GM) and right vastus lateralis (VLM) muscles of young team sports athletes, using volume (VC) and surface (SC) coils on a 3 T scanner, with the same receiver gain. Water spectra were used as internal reference for the absolute quantification of carnosine.
Results
Phantom’s experiments showed a maximum error of 7%, highlighting the validity of the measurements in the study setup. The carnosine concentrations (mmol/kg ww, mean ± SD) measured in the GM were 6.8 ± 2.2 with the VC (CcarVC) and 10.2 ± 3.0 with the SC (CcarSC) (P = 0.013; n = 9). Therefore, a correction was applied to these measurements (CcarVC = 0.6582*CcarSC), to make coils performance comparable (6.8 ± 2.2 for VC and 6.7 ± 2.0 for SC, P = 0.97). After that, only the SC was used to quantify carnosine in the VLM, where a concentration of 5.4 ± 1.5 (n = 30) was found, with significant differences between men (6.2 ± 1.3; n = 15) and women (4.6 ± 1.2; n = 15). The error in quantitation was 5.3–5.5% with both coils.
Conclusion
The method using the SC and water as internal reference can be used to quantify carnosine in voluminous muscles and regions of the body in humans, where the VC is not suitable, such as the VLM.
中文翻译:
使用表面线圈和水作为内部参考,使用 1H MRS 对股外侧肌中的肌肽进行体内绝对定量
客观的
使用表面线圈和水作为内部参考,对大腿肌肽的1 H MRS 肌内绝对定量方法进行标准化。
材料和方法
使用体积 (VC) 和表面 (SC) 在年轻团队运动运动员的体模(5、10 和 15 mM)以及右侧腓肠肌内侧肌 (GM) 和右侧股外侧肌 (VLM) 中获取肌肽光谱3 T 扫描仪上的线圈,具有相同的接收器增益。水光谱用作肌肽绝对定量的内部参考。
结果
Phantom 的实验显示最大误差为 7%,突出了研究设置中测量的有效性。在 GM 中测得的肌肽浓度(mmol/kg ww,平均值±标准偏差)在 VC(C汽车VC)中为 6.8 ± 2.2,在 SC(C汽车SC)中为 10.2 ± 3.0 ( P = 0.013;n = 9) . 因此,对这些测量值进行了校正(C汽车VC = 0.6582 * C汽车SC),以使线圈性能具有可比性(VC 为 6.8 ± 2.2,SC 为 6.7 ± 2.0,P = 0.97)。之后,仅使用 SC 来量化 VLM 中的肌肽,其中发现浓度为 5.4 ± 1.5(n = 30),男性之间存在显着差异(6.2 ± 1.3;n = 15) 和女性 (4.6 ± 1.2; n = 15)。两种线圈的定量误差均为 5.3–5.5%。
结论
使用 SC 和水作为内部参考的方法可用于量化人体大量肌肉和身体区域中的肌肽,其中 VC 不合适,例如 VLM。