Abstract

Pentachlorophenol (PCP) and astrafloxin FF (AF), a polymethine dye, were shown to form an ionic associate (IA), which could be rather perfectly extracted by different aromatic hydrocarbons. A maximum extraction of the ionic associate was attained at pH 8–12, being in quite a good agreement with the data obtained by calculating the distribution diagrams of corresponding dye and PCP forms. The distribution diagrams of these forms were calculated and constructed by using the MarvinScetch software. The effect of the dye concentration on the absorbance of the toluene extracts of PCP–AF ionic associates was studied. The PCP recovery attained a maximum at a dye concentration of (1.6–2.8) × 10^–4 M. Extraction equilibrium was established for 50–60 s. The stoichiometry of the PCP–AF ionic associate was studied by the spectrophotometric methods of isomolar series and equilibrium shift, and the ratio of components was 1 : 1. The scheme of the formation and extraction of this ionic associate was proposed. The conventional molar absorption coefficient of the ionic associate was 1.2 × 10⁵. The calibration curve of the extract absorbance as a function of the PCP concentration could be described by the linear equation А = 0.0121 + 0.0622c within a PCP concentration range of 1.2–60.3 μg/cm³. The PCP detection limit calculated by the 3s-criterion (n = 5, Р = 0.95) was 1.08 μg/cm³. The intralaboratory disperancy of the calibrating plot for PCP analysis with astrafloxin was estimated by the Cochran test G. The calculated Cochran test (n = 5, P = 0.95) was smaller than its tabular value: G_calcd = 0.27 < G_tab = 0.64 to argue for variance homogeneity. A method for the extraction photometric analysis of pentachlorophenol in water and bottom sediments was developed.

中文翻译：

---

用黄曲霉素萃取光度法分析水中五氯苯酚

摘要

五氯苯酚 (PCP) 和黄曲霉素 FF (AF)，一种聚甲炔染料，被证明可以形成离子缔合物 (IA)，可以相当完美地被不同的芳烃提取。离子缔合物的最大提取在 pH 8-12 时达到，与通过计算相应染料和 PCP 形式的分布图获得的数据非常吻合。使用MarvinScetch软件计算并构建了这些形态的分布图。研究了染料浓度对 PCP-AF 离子缔合物甲苯提取物吸光度的影响。^{PCP 回收率在 (1.6–2.8) × 10 –4}的染料浓度下达到最大值M. 萃取平衡建立 50-60 秒。采用等摩尔级数和平衡位移的分光光度法研究了PCP-AF离子缔合物的化学计量，组分的比例为1:1。提出了该离子缔合物的形成和提取方案。离子缔合物的常规摩尔吸收系数为1.2 × 10 ⁵。在 PCP 浓度范围为 1.2–60.3 μg/cm ³内，提取物吸光度随 PCP 浓度变化的校准曲线可以通过线性方程- = 0.0121 + 0.0622 c来描述。由 3 s标准计算的 PCP 检测限( n = 5, Р= 0.95) 为 1.08 μg/cm ³。通过 Cochran 检验G估计使用阿斯曲沙星进行 PCP 分析的校准图的实验室内分散度。计算出的 Cochran 检验 ( n = 5, P = 0.95) 小于其表格值：G _calcd = 0.27 < G _tab = 0.64 以证明方差同质性。建立了水和底泥中五氯苯酚的萃取光度分析方法。