Background

The present study intends to investigate the ability of gossypol-loaded casein nanoparticles (gossypol-loaded CAS NPs) for the treatment of ovarian cancer. The key emphasis of this study was to synthesize and characterize gossypol-embedded casein NPs by the desolvation technique.

Method

Casein is used as a polymer to fabricate the NPs; the fabricated nanoparticles are then characterized using UV–visible spectroscopy, FTIR spectroscopy, SEM, EDX, zeta potential and size analysis, and DSC to explore the efficiency and strength. The role of cross-linked casein nanoparticles was observed to improve the blood bioavailability of gossypol.

Results

The particle size of the optimized batch was found to be 278 ± 5 nm, and the PDI is 0.399; the zeta potential was found to be − 14.88 mV, and the % CDR of the optimized batch was found to be 55.66%. The cytotoxicity of gossypol-loaded CAS NPs was tested in vitro against a human breast cancer cell line (MCF-7) and found to be considerable.

Conclusions

The gossypol-loaded casein NPs were successfully synthesized with important advantages such as being easy to prepare, stable, and cost-effective with their applicability in ovarian cancer.

中文翻译：

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用于潜在靶向卵巢癌的棉酚包埋酪蛋白纳米颗粒：配方、表征和抗癌活性

背景

本研究旨在研究负载棉酚的酪蛋白纳米颗粒（负载棉酚的 CAS NPs）治疗卵巢癌的能力。本研究的重点是通过去溶剂化技术合成和表征棉酚包埋的酪蛋白 NPs。

方法

酪蛋白用作制造纳米粒子的聚合物；然后使用紫外-可见光谱、FTIR 光谱、SEM、EDX、zeta 电位和尺寸分析以及 DSC 对制造的纳米颗粒进行表征，以探索效率和强度。观察到交联酪蛋白纳米颗粒提高棉酚血液生物利用度的作用。

结果

优化批次的粒径为 278 ± 5 nm，PDI 为 0.399；zeta 电位为 - 14.88 mV，优化批次的 % CDR 为 55.66%。载有棉酚的 CAS NPs 的细胞毒性在体外针对人乳腺癌细胞系 (MCF-7) 进行了测试，发现其毒性相当大。

结论

成功合成了负载棉酚的酪蛋白纳米粒子，具有制备简单、稳定、成本低廉等重要优点，适用于卵巢癌。