There have been few findings on lipase synthesis from lactic acid bacteria (LAB) as compared to other categories of microorganisms. In this study, screening of lipase-producing lactobacilli from native dairy products was performed. Qualitative evaluation of lipolytic activity of lipase-producing lactobacilli was performed in different media. A clear zone observation around the colonies indicated the lipolytic activity. Among screened lipolytic bacterial strains, one sample (5c isolate) which showed the highest enzymatic activity was identified and confirmed by 16S rRNA gene sequence method with 98% identity to Lactobacillus fermentum (L. fermentum) under accession number of MH333208. Statistical experimental design including one-factor-at-a-time (OFAT) method was used to enhance the production of lipase by newly isolate of L. fermentum. NH₄Cl and temperature were the most influential parameters on lipase production and exhibited a positive influence among the twelve independent variables investigated in the Plackett–Burman (PB) design. The optimum values of three significant components influencing lipase production were determined by response surface methodology (RSM). The validity of the model developed was verified, and the optimum medium containing 4.23 g/l NH4Cl, temperature of 37.7 °C and 2% (v/v) inoculum size led to a maximum lipase production 1842.8 U/ml of culture medium which was 1.15 times above the unoptimized medium. These findings could be used for the future upscale lipase production using L. fermentum’s native isolate. Also, this work demonstrated the feasibility of statistical methodology to develop optimum medium composition for efficient lipase production using LAB isolates.

与其他类别的微生物相比，乳酸菌 (LAB) 合成脂肪酶的发现很少。在这项研究中，从天然乳制品中筛选产生脂肪酶的乳酸杆菌。在不同培养基中对产脂酶乳酸杆菌的脂解活性进行定性评价。菌落周围的清晰区域观察表明脂解活性。在筛选出的脂肪分解菌株中，通过 16S rRNA 基因序列法鉴定并确认了一个显示出最高酶活性的样品（5c 分离株）与发酵乳杆菌（L.发酵菌）具有 98% 的同一性) 登录号为 MH333208。包括一次一个因素（OFAT）方法的统计实验设计用于通过新分离的发酵乳杆菌来提高脂肪酶的产量。NH ₄Cl 和温度是对脂肪酶产生影响最大的参数，并且在 Plackett-Burman (PB) 设计中研究的 12 个自变量中表现出积极影响。通过响应面法 (RSM) 确定了影响脂肪酶生产的三个重要组分的最佳值。验证了所建立模型的有效性，含有 4.23 g/l NH4Cl、温度 37.7 °C 和 2% (v/v) 接种量的最佳培养基导致培养基的最大脂肪酶产量为 1842.8 U/ml。比未优化介质高 1.15 倍。这些发现可用于未来使用发酵乳杆菌的高档脂肪酶生产的原生隔离。此外，这项工作证明了使用 LAB 分离物开发用于高效生产脂肪酶的最佳培养基组成的统计方法的可行性。