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Optimization of Lipase Production by a Newly Isolate of Lactobacillus Fermentum

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Abstract

There have been few findings on lipase synthesis from lactic acid bacteria (LAB) as compared to other categories of microorganisms. In this study, screening of lipase-producing lactobacilli from native dairy products was performed. Qualitative evaluation of lipolytic activity of lipase-producing lactobacilli was performed in different media. A clear zone observation around the colonies indicated the lipolytic activity. Among screened lipolytic bacterial strains, one sample (5c isolate) which showed the highest enzymatic activity was identified and confirmed by 16S rRNA gene sequence method with 98% identity to Lactobacillus fermentum (L. fermentum) under accession number of MH333208. Statistical experimental design including one-factor-at-a-time (OFAT) method was used to enhance the production of lipase by newly isolate of L. fermentum. NH4Cl and temperature were the most influential parameters on lipase production and exhibited a positive influence among the twelve independent variables investigated in the Plackett–Burman (PB) design. The optimum values of three significant components influencing lipase production were determined by response surface methodology (RSM). The validity of the model developed was verified, and the optimum medium containing 4.23 g/l NH4Cl, temperature of 37.7 °C and 2% (v/v) inoculum size led to a maximum lipase production 1842.8 U/ml of culture medium which was 1.15 times above the unoptimized medium. These findings could be used for the future upscale lipase production using L. fermentum’s native isolate. Also, this work demonstrated the feasibility of statistical methodology to develop optimum medium composition for efficient lipase production using LAB isolates.

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Acknowledgements

This work was funded by the Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

    Foruzan Fathi & Zahra Moosavi-Nejad

  2. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

    Elahe Mobarak Qamsari, Rouha Kasra Kermanshahi & Tahereh Ghashghaei

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  1. Foruzan Fathi
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  2. Elahe Mobarak Qamsari
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  3. Rouha Kasra Kermanshahi
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  4. Zahra Moosavi-Nejad
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  5. Tahereh Ghashghaei
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Correspondence to Rouha Kasra Kermanshahi.

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Fathi, F., Mobarak Qamsari, E., Kasra Kermanshahi, R. et al. Optimization of Lipase Production by a Newly Isolate of Lactobacillus Fermentum. Iran J Sci Technol Trans Sci 46, 1103–1113 (2022). https://doi.org/10.1007/s40995-022-01322-5

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  • DOI: https://doi.org/10.1007/s40995-022-01322-5

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