In this study, active packaging films were developed by incorporating nano TiO₂ and pomegranate peel extract (PPE) into high-amylose corn starch (HCS)/konjac glucomannan (KG) matrix for the first time. The aim of present work was to investigate the influence of nano TiO₂ and PPE on physicochemical and functional properties of HCS/KG-based films. The films were characterized by XRD, FT-IR and SEM, and the tensile, antibacterial and antioxidant properties were evaluated. The results showed that the crystallinity of the composite films was increased and the microstructure was more uniform and dense after adding TiO₂ and PPE, and the intermolecular hydrogen bonds were formed between TiO₂, PPE, and film matrix. Incorporation of TiO₂ and PPE significantly improved tensile properties and decreased water solubility and water vapor permeability of the composite films (p < 0.05). HCS/KG films incorporated with TiO₂ and PPE presented remarkable antibacterial activity against Escherichia coli and Staphylococcus aureus, and exhibited strong antioxidant activity due to the polyphenol compounds in PPE. All films properties not only changed with the content of TiO₂ and PPE, but also improved synergistically when they were added together. The developed composite films were used as coating for the preservation of Agaricus bisporus, and the weight, firmness and total soluble solids of A. bisporus were significantly maintained and browning was effectively inhibited during storage. Therefore, HCS/KG composite film/coating containing TiO₂ and PPE has great potential as an attractive commercialization technology to ensure the quality and extend the shelf life of foods.

本研究首次将纳米TiO ₂和石榴皮提取物（PPE）掺入高直链玉米淀粉（HCS）/魔芋葡甘聚糖（KG）基质中，开发出活性包装薄膜。本工作的目的是研究纳米 TiO ₂和 PPE 对 HCS/KG 基薄膜的物理化学和功能特性的影响。采用XRD、FT-IR和SEM对薄膜进行表征，并评价其拉伸、抗菌和抗氧化性能。结果表明，加入TiO _{2和PPE后复合薄膜的结晶度提高，微观结构更加均匀致密，TiO 2}、PPE与薄膜基体之间形成了分子间氢键。加入二氧化钛₂和PPE显着改善了复合薄膜的拉伸性能，降低了水溶性和水蒸气渗透性（p <0.05）。含有TiO ₂和PPE的HCS/KG薄膜对大肠杆菌和金黄色葡萄球菌具有显着的抗菌活性，并且由于PPE中的多酚类化合物具有很强的抗氧化活性。所有薄膜的性能不仅随着TiO ₂和PPE的含量而改变，而且当它们一起添加时也协同改善。所研制的复合膜被用作双孢蘑菇的保鲜涂层，以及双孢蘑菇的重量、硬度和总可溶性固形物。在贮藏过程中显着保持，有效抑制褐变。_{因此，含有 TiO 2}和 PPE的 HCS/KG 复合薄膜/涂层作为一种有吸引力的商业化技术具有很大的潜力，可以确保食品的质量并延长食品的保质期。