Telenomus remus (Nixon) is a promising egg parasitoid for the management of Spodoptera frugiperda (J. E. Smith). This species has been successfully reared on alternative hosts under laboratory conditions. However, the production of biocontrol agents is often out of sync with the demands in the field. Appropriate cold storage techniques can drastically prolong their shelf-life to synchronize the release schedule with field needs and reduce production costs. Past studies on the cold storage of T. remus only focused on certain developmental stages, but not all stages. Here, we comprehensively evaluated the impacts of storage temperature (8, 11, and 14 °C) and duration (7, 14, 21, 28, and 35 days) on the maternal emergence and offspring fitness of T. remus stored at different developmental stages (first instar larvae, second instar larvae, prepupae, and pupae) using Spodoptera litura (Fabricius) eggs as alternative hosts. For each developmental stage, emergence percentage and parasitism capacity of parents all decreased with increased storage duration and decreased storage temperature. Maternal female longevity, offspring emergence percentage and percentage of females were barely affected by cold storage. We concluded that storage of the first instar larvae at 14 °C for 21 days was the optimum storage scheme for T. remus. Our findings can be directly used as guidance in mass production and storage of this parasitoid.

Telenomus remus (Nixon) 是一种很有前途的卵寄生蜂，用于管理草地贪夜蛾(JE Smith)。该物种已在实验室条件下在替代宿主上成功饲养。然而，生物防治剂的生产往往与该领域的需求不同步。适当的冷藏技术可以大大延长它们的保质期，使放行时间表与现场需求同步并降低生产成本。以往对大菱鲆冷藏的研究只集中在某些发育阶段，而不是所有阶段。在这里，我们综合评估了储藏温度（8、11 和 14 °C）和持续时间（7、14、21、28 和 35 天）对T. remus母体出现和后代适应性的影响使用斜纹夜蛾(Fabricius) 卵作为替代宿主，在不同发育阶段（一龄幼虫、二龄幼虫、预蛹和蛹）储存。对于每个发育阶段，亲本的出苗率和寄生能力均随着贮藏时间的延长和贮藏温度的降低而降低。母体雌性寿命、后代出苗率和雌性百分比几乎不受冷藏的影响。我们得出结论，将第一龄幼虫在 14°C 下储存 21 天是T. remus的最佳储存方案。我们的研究结果可以直接用作这种寄生蜂大规模生产和储存的指导。