当前位置: X-MOL 学术Eur. J. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Infection experiments of Pyrenophora teres f. maculata on cultivated and wild barley indicate absence of host specificity
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2022-03-21 , DOI: 10.1007/s10658-022-02496-9
Asieh Vasighzadeh 1 , Bahram Sharifnabi 1 , Mohammad Javan-Nikkhah 2 , Eva H. Stukenbrock 3, 4
Affiliation  

It is important to investigate the possibility of pathogen transmission between cultivated and uncultivated hosts due to the role of the latter in pathogen evolution and the creation of new pathotypes which may break resistance genes of cultivated hosts. Wild hosts can also act as a pathogen reservoir offseason and cause pathogen survival. Spot form of net blotch (SFNB), caused by the fungus Pyrenophora teres f. maculata (PTM), is an important foliar disease of barley worldwide. In this study, 19 isolates from barley and Hordeum murinum were identified as P. teres based on ITS regions and gpd sequence and 17 of these isolates were identified as the sub-species PTM based on PCR assay. In order to evaluate the pathogenicity of PTM isolates obtained from H. murinum on barley as well barley PTM isolates on H. murinum, three barley isolates and two H. murinum isolates were inoculated on one H. murinum line and four barley cultivars including Local, Jolge, Zahak and Oksin, which were previously identified as sensitive, semi-sensitive, semi-resistant and resistant to PTM, respectively. The net blotch severity was scored based on a 1–9 scale. ANOVA showed that interaction between hosts and isolates was not different significantly (Fisher’s test, P = 0.05) which means that each isolate had the same pathogenic behavior on both barley and H. murinum. Therefore, it is possible to transfer pathogens from wild barley to barley as well as in the opposite direction and H. murinum can be considered a threat to barley because of its potential as a PTM reservoir between two growing seasons as well as creating new pathotypes.



中文翻译:

Pyrenophora teres f 的感染实验。栽培和野生大麦上的斑块表明缺乏宿主特异性

由于后者在病原体进化中的作用以及可能破坏培养宿主抗性基因的新致病型的产生,因此研究培养和未培养宿主之间病原体传播的可能性非常重要。野生宿主也可以作为病原体库淡季并导致病原体存活。由真菌Pyrenophora teres f引起的斑点状网斑 (SFNB) 。斑斑病(PTM)是世界范围内一种重要的大麦叶面病害。在这项研究中,基于 ITS 区域和gpd序列,从大麦和Hordeum murinum中分离出的 19 株被鉴定为P. teres ,其中 17 株被鉴定为PTM亚种。基于PCR测定。为了评估从H. murinum获得的PTM分离物对大麦以及大麦PTM分离物对H. murinum的致病性,将三个大麦分离物和两个H. murinum分离物接种到一个H. murinum品系和四个大麦品种,包括 Local, Jolge、Zahak 和 Oksin,之前被确定为对PTM敏感、半敏感、半耐药和耐药, 分别。基于 1-9 等级对净斑点严重程度进行评分。ANOVA 显示宿主和分离物之间的相互作用没有显着差异(Fisher 检验,P = 0.05),这意味着每种分离物对大麦和H.murinum具有相同的致病行为。因此,有可能将病原体从野生大麦转移到大麦以及以相反的方向传播,而H. murinum可以被认为是对大麦的威胁,因为它有可能成为两个生长季节之间的PTM库以及产生新的致病型。

更新日期:2022-03-21
down
wechat
bug