Abstract
It is important to investigate the possibility of pathogen transmission between cultivated and uncultivated hosts due to the role of the latter in pathogen evolution and the creation of new pathotypes which may break resistance genes of cultivated hosts. Wild hosts can also act as a pathogen reservoir offseason and cause pathogen survival. Spot form of net blotch (SFNB), caused by the fungus Pyrenophora teres f. maculata (PTM), is an important foliar disease of barley worldwide. In this study, 19 isolates from barley and Hordeum murinum were identified as P. teres based on ITS regions and gpd sequence and 17 of these isolates were identified as the sub-species PTM based on PCR assay. In order to evaluate the pathogenicity of PTM isolates obtained from H. murinum on barley as well barley PTM isolates on H. murinum, three barley isolates and two H. murinum isolates were inoculated on one H. murinum line and four barley cultivars including Local, Jolge, Zahak and Oksin, which were previously identified as sensitive, semi-sensitive, semi-resistant and resistant to PTM, respectively. The net blotch severity was scored based on a 1–9 scale. ANOVA showed that interaction between hosts and isolates was not different significantly (Fisher’s test, P = 0.05) which means that each isolate had the same pathogenic behavior on both barley and H. murinum. Therefore, it is possible to transfer pathogens from wild barley to barley as well as in the opposite direction and H. murinum can be considered a threat to barley because of its potential as a PTM reservoir between two growing seasons as well as creating new pathotypes.
References
Akhavan, A., Turkington, T. K., Askarian, H., Tekauz, A., Xi, K., Tucker, J. R., Kutcher, H. R., & Strelkov, S. E. (2016). Virulence of Pyrenophora teres populations in western Canada. Canadian Journal of Plant Pathology, 38(2), 183–196. https://doi.org/10.1080/07060661.2016.1159617
Backes, A., Guerriero, G., Barka, E. A., & Jacquard, C. (2021). Pyrenophora teres: Taxonomy, morphology, interaction with barley, and mode of control. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.614951
Berbee, M. L., Pirseyedi, M., & Hubbard, S. (1999). Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia, 91(6), 964–977. https://doi.org/10.1080/00275514.1999.12061106
Brown, M. P., Steffenson, B. J., & Webster, R. K. (1993). Host range of Pyrenophra teres f. teres isolates from California. Plant Disease (USA), 77, 942–947. https://doi.org/10.1094/PD-77-0942
Burdon, J. J., & Thrall, P. H. (2008). Pathogen evolution across the agro-ecological interface: Implications for disease management. Evolutionary Applications, 1(1), 57–65. https://doi.org/10.1111/j.1752-4571.2007.00005.x
Ershad D.2009–Fungi of Iran. 3rd edition, Iranian research institution of plant protection, 531p.
Fowler, R. A., Platz, G. J., Bell, K. L., Fletcher, S. E. H., Franckowiak, J. D., & Hickey, L. T. (2017). Pathogenic variation of Pyrenophora teres f. teres in Australia. Australasian Plant Pathology, 46(2), 115–128. https://doi.org/10.1007/s13313-017-0468-1
Ghazvini, H. O., Kohkan, S. A., Lakzadeh, I., Fallahi, H. A., Alt Jafarbay, J., Ghasemi, M., et al. (2014). Zahak, a New Irrigated Barley Cultivar with Wide Adaptability in the Warm and Dry Agro-Climate Zone in the South of Iran. Research Achievements for Field and Horticulture Crops, 3(1), 15–26. https://doi.org/10.22092/rafhc.2014.100297
Ghazvini, H., Koocheki, A. R., Yousefi, A., Razavi, S. A., Mohammadi, S., Aminzade, G. R., et al. (2017). Jolge, a new irrigated barley cultivar with wide adaptability in the cold agro-climate zone of Iran. Research Achievements for Field and Horticulture Crops, 6(1), 37–49. https://doi.org/10.22092/rafhc.2018.107771.1042
Ghazvini, H., Lakzade, I., Kouhkan, S. A., Jabari, M., Barati, A., Fallahi, H. A., et al. (2019). Oksin, a new irrigated six-rowed barley cultivar with wide adaptability in warm agro-climate zone of Iran. Research Achievements for Field and Horticulture Crops, 7(2), 149–159. https://doi.org/10.22092/rafhc.2019.121086.1125
Hennessy, C., Walduck, G., Daly, A., & Padovan, A. (2005). Weed hosts of fusarium oxysporum f. sp. cubense tropical race 4 in northern Australia. Australasian Plant Pathology, 34(1), 115–117. https://doi.org/10.1071/AP04091
Jenkinson, N. P., & Parry, D. W. (1994). Isolation of fusarium species from common broad-leaved weeds and their pathogenicity to winter wheat. Mycological Research, 98(7), 776–780. https://doi.org/10.1016/S0953-7562(09)81054-X
Kenneth, R. (1962). On the taxonomy, morphology and geographic origins of Pyrenophora teres Drechsler and allied species. Bulletin of the Research Council of Israel, 11, 55–82.
Khan, T. N. (1973). Host specialization by Western Australian isolates causing net blotch symptoms on Hordeum. Transactions of the British Mycological Society, 61(2), 215–220. https://doi.org/10.1016/S0007-1536(73)80144-5
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870–1874. https://doi.org/10.1093/molbev/msw054
Linde, C. C., & Smith, L. M. (2019). Host specialisation and disparate evolution of Pyrenophora teres f. teres on barley and barley grass. BMC Evolutionary Biology, 19(1), 1–14. https://doi.org/10.1186/s12862-019-1446-8
Linde, C. C., Smith, L. M., & Peakall, R. (2016). Weeds, as ancillary hosts, pose disproportionate risk for virulent pathogen transfer to crops. BMC Evolutionary Biology, 16(1), 1–12. https://doi.org/10.1186/s12862-016-0680-6
Liu, Z., Ellwood, S. R., Oliver, R. P., & Friesen, T. L. (2011). Pyrenophora teres: Profile of an increasingly damaging barley pathogen. Molecular Plant Pathology, 12(1), 1–19. https://doi.org/10.1111/j.1364-3703.2010.00649.x
Louw, J. P. J., Crous, P. W., & Holz, G. (1996). Relative importance of the barley net blotch pathogens Pyrenophora teres f. teres (net-type) and P. teres f. maculata (spot-type) in South Africa. African Plant Protection, 2(2), 89–95. https://doi.org/10.1111/J.1439-0434.1995.TB00245.X
Lu, S., Platz, G. J., Edwards, M. C., & Friesen, T. L. (2010). Mating type locus-specific polymerase chain reaction markers for differentiation of Pyrenophora teres f. teres and P. teres f. maculata, the causal agents of barley net blotch. Phytopathology, 100(12), 1298–1306. https://doi.org/10.1094/PHYTO-05-10-0135
Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19), 4321–4326. https://doi.org/10.1093/nar/8.19.4321
Oğuz, A. Ç., & Karakaya, A. (2021). Genetic diversity of barley foliar fungal pathogens. Agronomy, 11(3), 434 https://www.mdpi.com/2073-4395/11/3/434/htm
Oğuz, A. Ç., Ölmez, F., & Karakaya, A. (2019). Genetic diversity of net blotch pathogens of barley in Turkey. International Journal of Agriculture and Biology. https://doi.org/10.3390/agronomy11030434
Ronen, M., Sela, H., Fridman, E., Perl-Treves, R., Kopahnke, D., Moreau, A., Ben-David, R., & Harel, A. (2019). Characterization of the barley net blotch pathosystem at the center of origin of host and pathogen. Pathogens, 8(4), 275. https://doi.org/10.3390/pathogens8040275
Seifollahi, E., Sharifnabi, B., Javan-Nikkhah, M., & Linde, C. C. (2020). Scald on gramineous hosts in Iran and their potential threat to cultivated barley. Mycological Progress, 19(3), 223–233. https://doi.org/10.1111/ppa.12886
Shipton, W. A., Khan, T. N., & Boyd, W. J. R. (1973). Net blotch of barley. Review of Plant Pathology., 52, 269–290.
Tamura, K., & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10(3), 512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
Tekauz, A. (1985). A numerical scale to classify reactions of barley to Pyrenophora teres. Canadian Journal of Plant Pathology, 7(2), 181–183. https://doi.org/10.1080/07060668509501499
Vasighzadeh, A., Sharifnabi, B., Javan-Nikkhah, M., Seifollahi, E., Landermann-Habetha, D., Feurtey, A., & Holtgrewe-Stukenbrock, E. (2021). Population genetic structure of four regional populations of the barley pathogen Pyrenophora teres f. maculata in Iran is characterized by high genetic diversity and sexual recombination. Plant Pathology, 70(3), 735–744. https://doi.org/10.1111/ppa.13326
Wellings, C. R. (2007). Puccinia striiformis in Australia: A review of the incursion, evolution, and adaptation of stripe rust in the period 1979–2006. Australian Journal of Agricultural Research, 58(6), 567–575. https://doi.org/10.1071/ar07130
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Acknowledgements
A.V. thanks Isfahan University of Technology for financial support. The authors thank S. Taghadomi-Saberi who contribute during sampling and E. Seifollahi for guidance in pathogenicity test. This work was funded by a stipend to A.V. from the Iranian Ministry of Science, Research and Technology (MSRT) and Iran National Science Foundation (INSF).
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Vasighzadeh, A., Sharifnabi, B., Javan-Nikkhah, M. et al. Infection experiments of Pyrenophora teres f. maculata on cultivated and wild barley indicate absence of host specificity. Eur J Plant Pathol 163, 749–759 (2022). https://doi.org/10.1007/s10658-022-02496-9
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DOI: https://doi.org/10.1007/s10658-022-02496-9