Conventional breeding and genetics in a vegetatively propagated, autotetraploid, highly heterozygous recalcitrant crop species such as potato is challenging. Diploid inbred lines in potato will serve as an invaluable source of material for breeding and functional genetics. Most of the available diploid germplasm in potato is either self-incompatible and/or is not amenable to genetic engineering. Traits including self-compatibility, tuber quality and amenability to genetic transformation are critical for current potato breeding and genetics. This study evaluates selected diploid potato germplasm from various breeding programs in the United States including 1S1, DMRH-S5-28–5, M6, MSEE720-04, MSEE737-05, MSEE824-04, and UW-W4 for the above traits. Our results indicate that diploid lines 1S1, MSEE737-05 and UW-W4 have tissue culture regeneration efficiency of over 75%, are amenable to Agrobacterium-mediated genetic transformation, produce viable fruit upon selfing and form tubers with desirable appearances.

无性繁殖、同源四倍体、高度杂合的顽固作物物种（如马铃薯）的常规育种和遗传学具有挑战性。马铃薯中的二倍体自交系将作为育种和功能遗传学的宝贵材料来源。马铃薯中大多数可用的二倍体种质要么是自交不亲和的，要么不适合基因工程。包括自交亲和性、块茎质量和遗传转化顺从性在内的性状对于当前的马铃薯育种和遗传学至关重要。本研究针对上述性状评估了从美国各种育种计划中选择的二倍体马铃薯种质，包括 1S1、DMRH-S5-28-5、M6、MSEE720-04、MSEE737-05、MSEE824-04 和 UW-W4。我们的结果表明，二倍体系 1S1，农杆菌介导的遗传转化，在自交时产生有活力的果实并形成具有理想外观的块茎。