Abstract
Conventional breeding and genetics in a vegetatively propagated, autotetraploid, highly heterozygous recalcitrant crop species such as potato is challenging. Diploid inbred lines in potato will serve as an invaluable source of material for breeding and functional genetics. Most of the available diploid germplasm in potato is either self-incompatible and/or is not amenable to genetic engineering. Traits including self-compatibility, tuber quality and amenability to genetic transformation are critical for current potato breeding and genetics. This study evaluates selected diploid potato germplasm from various breeding programs in the United States including 1S1, DMRH-S5-28–5, M6, MSEE720-04, MSEE737-05, MSEE824-04, and UW-W4 for the above traits. Our results indicate that diploid lines 1S1, MSEE737-05 and UW-W4 have tissue culture regeneration efficiency of over 75%, are amenable to Agrobacterium-mediated genetic transformation, produce viable fruit upon selfing and form tubers with desirable appearances.
Resumen
La reproducción convencional y la genética en una especie de cultivo recalcitrante altamente heterocigota, autotetraploide y propagada vegetativamente, como la papa, es un desafío. Las líneas endogámicas diploides en la papa se convertirán en una fuente invaluable de material para mejoramiento y análisis funcionales. La mayor parte del germoplasma diploide disponible en la papa es autoincompatinable y / o no es susceptible a la transformación genética. Los rasgos que incluyen la autocompatibilidad, la calidad del tubérculo y la capacidad de transformación genética son críticos para el mejoramiento y la genética actuales de la papa. Este estudio evalúa germoplasma de papa diploide seleccionado de varios programas de mejoramiento en los Estados Unidos, incluidos 1S1, DMRH-S5-28–5, M6, MSEE720-04, MSEE737-05, MSEE824-04 y UW-W4 para los rasgos mencionados anteriormente. Nuestros resultados indican que las líneas diploides 1S1, MSEE737-05 y UW-W4, los cuales tienen una eficiencia de regeneración de cultivo de tejidos de más del 75%, son susceptibles de transformación genética mediada por Agrobacterium, producen frutos viables al ser autopolinizadas y forman tubérculos con apariencias deseables.
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Acknowledgements
The authors would like to thank Gabriela Massa for guidance on methods for regeneration and Maher Alsahlany for developing diploid germplasm for MSU’s potato breeding program used in this study. They would also like to thank Dr. Courtney Hollender and Dr. C.Robin Buell at Michigan State University for use of the Nikon Eclipse Ni-u microscope and Leica DM750 microscope, respectively. The research conducted complies with current laws and regulations of the United States. Funding for this study was provided by the Biotechnology Risk Assessment Grant Program competitive grant no. 2018-33522- 28736 from the USDA National Institute of Food and Agriculture and the Agricultural Research Service.
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Supplementary Table 1.
Average total tuber yield from greenhouse plants (XLSX 10 kb)
Supplementary Fig. 1.
Antibiotic lethality dosages for a) internode and b) leaf explants of 1S1 using kanamycin, hygromycin and G418 after 2 weeks on selection regeneration media. Internode and leaf explants for 1S1 remain green and healthy for even as high as 150 mg/L of kanamycin, exhibiting resistance to this antibiotic. On hygromycin media, as low as 20 mg/L and 10 mg/L is lethal to internode and leaf explants, respectively. On G418 media, the lethality dose is 30 mg/L for both internode and leaf explants. (PNG 7711 kb)
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Jayakody, T.B., Enciso-Rodríguez, F.E., Jensen, J. et al. Evaluation of diploid potato germplasm for applications of genome editing and genetic engineering. Am. J. Potato Res. 99, 13–24 (2022). https://doi.org/10.1007/s12230-021-09855-y
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DOI: https://doi.org/10.1007/s12230-021-09855-y