DNA manipulation is crucial for many biotechnological prospects and for medical applications such as gene therapy. This requires the amplification and extraction of DNA from bacteria and the transfer of these DNA molecules into cells, including bacterial and mammalian cells. The capacity of the natural magnesium silicate clay mineral sepiolite to bind to DNA makes it a potentially useful tool for biotechnological/medical strategies. In addition, sepiolite is inexpensive and classified as non-toxic and non-carcinogenic. This review will first describe the physicochemical interactions between sepiolite and DNA. Then, the leverage of sepiolite/DNA interactions for DNA extraction from bacteria, to optimize DNA transfer into bacteria and DNA transfection into mammalian cells, are presented. Finally, the putative toxicity of sepiolite and its advantages and perspectives for future prospects, such as the improvement of immunotherapy, are also discussed.

DNA 操作对于许多生物技术前景和基因治疗等医学应用至关重要。这需要从细菌中扩增和提取 DNA，并将这些 DNA 分子转移到细胞中，包括细菌和哺乳动物细胞。天然硅酸镁粘土矿物海泡石与 DNA 结合的能力使其成为生物技术/医学策略的潜在有用工具。此外，海泡石价格低廉，被归类为无毒无致癌物。本综述将首先描述海泡石和 DNA 之间的物理化学相互作用。然后，介绍了利用海泡石/DNA 相互作用从细菌中提取 DNA，以优化 DNA 向细菌的转移和 DNA 向哺乳动物细胞的转染。最后，