Abstract
DNA manipulation is crucial for many biotechnological prospects and for medical applications such as gene therapy. This requires the amplification and extraction of DNA from bacteria and the transfer of these DNA molecules into cells, including bacterial and mammalian cells. The capacity of the natural magnesium silicate clay mineral sepiolite to bind to DNA makes it a potentially useful tool for biotechnological/medical strategies. In addition, sepiolite is inexpensive and classified as non-toxic and non-carcinogenic. This review will first describe the physicochemical interactions between sepiolite and DNA. Then, the leverage of sepiolite/DNA interactions for DNA extraction from bacteria, to optimize DNA transfer into bacteria and DNA transfection into mammalian cells, are presented. Finally, the putative toxicity of sepiolite and its advantages and perspectives for future prospects, such as the improvement of immunotherapy, are also discussed.
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16 December 2021
This article was updated to correct errors introduced during the production process.
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ACKNOWLEDGMENTS
The authors thank Prof. Eduardo Ruiz-Hiztky and Dr Pilar Aranda for sharing their expertise, particularly on sepiolite, and for stimulating discussions. OP acknowledges Dr Eric Le Cam for access to the electron microscope and helpful discussions. SR and BSL were supported by grants from the Ligue Nationale contre le cancer “Equipe labellisée”, ANR (Agence Nationale de la Recherche, ANR-16-CE12-0011-02, and ANR-16-CE18-0012-02), and INCa (Institut National du Cancer, PLBIO18-232). OP was supported by the CNRS Mission pour l’Interdisciplinarité (MI-DynAFM-DNARep 2018_273085) and the Bourgogne Franche-Comté Graduate School EUR-EIPHI (17-EURE-0002).
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This paper belongs to a special issue on ‘Clay Minerals in Health Applications’
(Received 6 May 2021; revised 25 October 2021; AE: Youjun Deng)
Sandrine Ragu and Olivier Piétrement contributed equally to the writing of this paper
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Ragu, S., Piétrement, O. & Lopez, B.S. BINDING OF DNA TO NATURAL SEPIOLITE: APPLICATIONS IN BIOTECHNOLOGY AND PERSPECTIVES. Clays Clay Miner. 69, 633–640 (2021). https://doi.org/10.1007/s42860-021-00165-2
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DOI: https://doi.org/10.1007/s42860-021-00165-2