Purpose

Xijiao Dihuang Tang (XJDHT) is a classical formula of traditional Chinese medicine constituted of Cornu Bubali, Rehmannia glutinosa (Gaertn.) DC., Paeonia lactiflora Pall., and Paeonia suffruticosa Andrews. It was first mentioned in the medical classic “Beiji Qianjin Yaofang” written by Simiao Sun in Tang Dynasty. It shows very strong antipyretic and anticoagulant effects and has been clinically applied to treat various type of blood loss, purple and black spots, heat stroke, and glossitis. Kawasaki disease (KD) is considered as a kind of acute febrile illness in children with systemic vasculitis as the main lesions. The aim of this research is to clarify whether XJDHT can play a protective role in KD.

Methods

A mouse model of Candida albicans water-soluble fraction (CAWS)–induced coronary arteritis and a KD cell model with tumor necrosis factor (TNF)-α induction were employed to investigate the potential effect and mechanism of XJDHT on coronary artery injury in KD.

Results

Data showed that XJDHT remarkably alleviated the coronary artery injury of KD mice, as evidenced by reduced inflammation and downregulated expression of pro-inflammatory cytokines interleukin (IL)-1β and TNF-α. In vitro investigation showed that XJDHT could promote cell proliferation, inhibit cell apoptosis, and improve mitochondrial functions. Subsequent studies demonstrated that XJDHT rescued endothelial cell injury by PI3K/Akt-NFκB signaling pathway. Component analysis of XJDHT detected thirty-eight chemically active ingredients, including paeoniflorin, albiflorin, and paeoniflorigenone, which in in vitro experiments exhibited significant rescue effects on TNF-α–mediated endothelial cell injury.

Conclusion

Our findings demonstrated that XJDHT mitigated coronary artery injury of KD through suppressing endothelial cell damage via PI3K/Akt-NFκB signaling.

中文翻译：

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西胶地黄汤对川崎病冠状动脉损伤的保护作用

目的

西胶地黄汤 (XJDHT) 是由水牛角、地黄(Gaertn.) DC.、Paeonia lactiflora Pall. 和Paeonia suffruticosa Andrews组成的中药经典方剂。最早见于唐代孙思邈所著的医学经典《北极千金要方》。具有很强的解热、抗凝血作用，临床上用于治疗各种失血、紫斑、中暑、舌炎等。川崎病(KD)被认为是一种以全身性血管炎为主要病变的小儿急性发热性疾病。本研究的目的是阐明 XJDHT 是否可以在 KD 中发挥保护作用。

方法

采用白色念珠菌水溶性组分 (CAWS) 诱导的冠状动脉炎小鼠模型和肿瘤坏死因子 (TNF)-α 诱导的 KD 细胞模型，研究 XJDHT 对 KD 冠状动脉损伤的潜在作用和机制。

结果

数据显示，XJDHT 显着减轻了 KD 小鼠的冠状动脉损伤，炎症减轻和促炎细胞因子白细胞介素 (IL)-1β 和 TNF-α 的表达下调证明了这一点。体外研究表明，XJDHT 可以促进细胞增殖，抑制细胞凋亡，改善线粒体功能。随后的研究表明，XJDHT 通过 PI3K/Akt-NFκB 信号通路挽救内皮细胞损伤。XJDHT的成分分析检测出芍药甙、白花甙、芍药苷酮等38种化学活性成分，在体外实验中对TNF-α介导的内皮细胞损伤具有显着的修复作用。

结论

我们的研究结果表明，XJDHT 通过 PI3K/Akt-NFκB 信号传导抑制内皮细胞损伤，从而减轻了 KD 的冠状动脉损伤。