Journal of Inorganic and Organometallic Polymers and Materials ( IF 3.9 ) Pub Date : 2021-10-19 , DOI: 10.1007/s10904-021-02128-5 J. Saranya 1 , B. S. Sreeja 2 , K. Bhuvaneswari 2 , M. Arivanandan 3 , S. Sherin 4 , K. S. Shivani 4 , G. SaradhaPreetha 4 , K. K. Saroja 4
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Using the ultrasonic approach, we produced a morphology involving a cerium oxide/ zinc oxide/graphene oxide nanocomposite-based system. The developed nanocomposite was examined using X-Ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The average crystallite size was found to be 11.44 nm, as determined by XRD. An FTIR analysis was used to confirm the existence of functional groups. FESEM was used to verify the morphological properties of CeO2/ZnO/GO. The micromorphology of the CeO2/ZnO/GO nanocomposite reveals a smoother sheet-like structure. In addition, using an antiproliferative assay test, the developed nanosystem was evaluated for its scavenging anti-cancer capability against HeLa cell lines in various doses at various incubation intervals. In our investigation, the effective IC50 concentration was reported to be 62.5 µg/ml at 72 h. Further, the developed nanosystem was evaluated for its killing efficacy against a normal cell line. To identify the apoptosis-associated alterations of cell membranes throughout the apoptosis process, a dual acridine orange/ethidium bromide (AO/EB) fluorescent staining was done using the CeO2/ZnO/GO nanocomposite in three specific concentrations. The quantitative analysis was carried out using flow cytometry (FACS study) to determine the cell cycle during which the greatest number of HeLa cells were destroyed. According to the results of the FACS investigation, the maximum cell cycle has taken place in P2, P4.As a result, the newly designed CeO2/ZnO/GO hybrid has demonstrated improved anti-cancer efficacy against the HeLa cell line, making it a better therapeutic agent for cervical cancer detection.
Graphic abstract
中文翻译：
用于评估 HeLa 和 VERO 细胞系细胞毒性和凋亡行为的氧化铈/氧化锌/氧化石墨烯复合材料的纳米结构
使用超声波方法，我们产生了涉及氧化铈/氧化锌/氧化石墨烯纳米复合材料系统的形态。使用 X 射线衍射、傅里叶变换红外光谱和场发射扫描电子显微镜检查开发的纳米复合材料。经XRD测定，发现平均微晶尺寸为11.44nm。FTIR 分析用于确认官能团的存在。FESEM用于验证CeO 2 /ZnO/GO的形态特性。CeO 2的微观形貌/ZnO/GO 纳米复合材料显示出更光滑的片状结构。此外，使用抗增殖试验，评估了开发的纳米系统在不同孵育时间间隔内对不同剂量的 HeLa 细胞系的清除抗癌能力。在我们的调查中，据报道有效 IC50 浓度在 72 小时时为 62.5 µg/ml。此外，还评估了开发的纳米系统对正常细胞系的杀伤功效。为了在整个细胞凋亡过程中鉴定细胞膜与细胞凋亡相关的改变，使用三种特定浓度的 CeO2/ZnO/GO 纳米复合材料进行双重吖啶橙/溴化乙锭 (AO/EB) 荧光染色。使用流式细胞术（FACS 研究）进行定量分析，以确定最多数量的 HeLa 细胞被破坏的细胞周期。根据 FACS 调查的结果，最大的细胞周期发生在 P2、P4。因此，新设计的 CeO2/ZnO/GO 杂化物对 HeLa 细胞系具有更高的抗癌功效，使其成为一种更好的宫颈癌检测治疗剂。
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