Signal-mediated regulation of protein trafficking is an elegant mechanism for controlling the delivery of molecules to a precise location for critical signaling events that occur over short time frames. During plant reproduction, the FERONIA receptor complex is critical for intercellular communication that leads to gamete delivery; however, the impact of the FERONIA signal transduction cascade on protein trafficking in synergid cells remains unknown. Live imaging of pollen tube reception has revealed that a key outcome of FERONIA signaling is polar accumulation of the MLO protein NORTIA at the filiform apparatus in response to signals from an arriving pollen tube. Artificial delivery of NORTIA to the filiform apparatus is sufficient to bypass the FERONIA signaling pathway and to promote interspecific pollen tube reception. We propose that polar accumulation of NORTIA leads to the production of a secondary booster signal to ensure that pollen tubes burst to deliver the sperm cells for double fertilization.

信号介导的蛋白质运输调节是一种优雅的机制，用于控制分子向精确位置的传递，以便在短时间内发生关键信号事件。在植物繁殖过程中，FERONIA 受体复合物对于导致配子传递的细胞间通讯至关重要；然而，FERONIA 信号转导级联对协同细胞中蛋白质运输的影响仍然未知。花粉管接收的实时成像显示，FERONIA 信号传导的一个关键结果是 MLO 蛋白 NORTIA 在丝状装置的极性积累，以响应来自到达的花粉管的信号。人工递送 NORTIA 到丝状装置足以绕过 FERONIA 信号通路并促进种间花粉管接收。