Polarized NORTIA accumulation in response to pollen tube arrival at synergids promotes fertilization

Highlights

• Pollen tube arrival induces NTA trafficking from Golgi to filiform apparatus

• Polar accumulation of NTA at filiform apparatus (faNTA) is necessary for function

• faNTA is sufficient to bypass FERONIA and LORELEI signaling

• faNTA promotes interspecific pollen tube reception

Summary

Signal-mediated regulation of protein trafficking is an elegant mechanism for controlling the delivery of molecules to a precise location for critical signaling events that occur over short time frames. During plant reproduction, the FERONIA receptor complex is critical for intercellular communication that leads to gamete delivery; however, the impact of the FERONIA signal transduction cascade on protein trafficking in synergid cells remains unknown. Live imaging of pollen tube reception has revealed that a key outcome of FERONIA signaling is polar accumulation of the MLO protein NORTIA at the filiform apparatus in response to signals from an arriving pollen tube. Artificial delivery of NORTIA to the filiform apparatus is sufficient to bypass the FERONIA signaling pathway and to promote interspecific pollen tube reception. We propose that polar accumulation of NORTIA leads to the production of a secondary booster signal to ensure that pollen tubes burst to deliver the sperm cells for double fertilization.