Non-thermal plasma is widely considered as an effective technology for applications in agriculture. Particularly, numerous reports studies have highlighted the role of plasma-activated water (PAW) for seeds germination, plant growth, stress tolerance, and antibacterial ability. The present study investigated the effects of PAW on lettuce (Lactuca Sativa L.) seed germination and seedling growth. PAW was achieved by using an atmospheric pressure dielectric barrier discharge in Ar (50%) –N₂ (40%)–O₂ (10%) gas mixture for treatment time ranging from 5 to 30 mn. The physicochemical properties of PAW (temperature, pH, electrical conductivity, concentrations of nitrate, nitrite, and hydrogen peroxide) were evaluated. Results show that water-activated during moderate time, 10 to 20 mn, contains reactive oxygen and nitrogen species at relevant concentration levels to have active impacts on seed germination and seedling growth. Germination potential significantly increased by about 117%, 56%, and 77% after 15 mn of treatment, for the first 3 days, respectively, compared to control. For long time PAW (25–30 mn), the germination rate is either constant or decreases. Positive effects of PAW treatments were registered on the growth parameters of seedling including stem and root length, leaf weight, leaf area and chlorophyll content, and the vigor of seedlings. Chlorophyll content significantly increased by 220% for PAW-treated 15 mn and by about 165% for PAW-treated 20 mn, respectively, compared with control. Additionally, PAW induce morphological changes on lettuce seeds which are associated with oxidizing species leading to better water and nutrients uptake.

非热等离子体被广泛认为是一种在农业中应用的有效技术。特别是，大量报告研究强调了等离子体活化水 (PAW) 在种子萌发、植物生长、胁迫耐受性和抗菌能力中的作用。本研究调查了 PAW 对生菜 ( Lactuca Sativa L. ) 种子萌发和幼苗生长的影响。PAW 是通过在 Ar (50%) –N ₂ (40%)–O _{2 中}使用大气压介质阻挡放电实现的(10%) 气体混合物，处理时间为 5 至 30 百万分钟。评估了 PAW 的理化特性（温度、pH、电导率、硝酸盐、亚硝酸盐和过氧化氢的浓度）。结果表明，在中等时间（10 至 20 百万年）的水活化含有相关浓度水平的活性氧和氮物质，对种子萌发和幼苗生长产生积极影响。与对照相比，在前 3 天处理 1500 万后，发芽潜力分别显着增加了约 117%、56% 和 77%。对于长时间的 PAW (25–30 mn)，发芽率要么保持不变，要么下降。PAW 处理对幼苗的生长参数有积极影响，包括茎和根长度、叶重、叶面积和叶绿素含量，和幼苗的活力。与对照相比，经 PAW 处理的 1500 万叶绿素含量分别显着增加了 220%，经 PAW 处理的 2000 万叶叶绿素含量分别显着增加了约 165%。此外，PAW 诱导生菜种子的形态变化，这与氧化物种有关，从而导致更好的水分和养分吸收。