Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.

中文翻译：

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重新利用 Hybrid Capture 2 (HC2) 筛选测试对人乳头瘤病毒进行全基因组测序。

需要通过下一代测序对人乳头瘤病毒 (HPV) 进行基因组分析的简单和标准化方法。该研究的目的是基于广泛使用的商业 Hybrid Capture 2 (QIAGEN) 测试，开发一种直接深度测序高风险 (hr) HPV 毒株的协议，无需任何额外的探针设计。该协议适用于 15 个 HPV 阳性和两个 HPV 阴性宫颈样本或细胞系，并通过将测序结果与使用商业基因分型试剂盒获得的结果进行比较，在基因型水平上进行验证。在这项原理验证研究中提出的我们协议的性能支持其用于准确表征 hrHPV 的遗传变异。