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Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses

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Abstract

Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.

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Acknowledgements

We thank Professor Paul Lambert for kindly providing us with W12 cells.

Funding

Part of this work was funded by the Région Franche-Comté. AD is a recipient of a predoctoral scholarship from the Ministère de l'Enseignement Supérieur, de la Recherche et de l'Innovation.

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Correspondence to Q. Lepiller.

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None declared

Ethical approval

All samples were stored in a biobank for which a declaration of preparation and storage of human samples for research use has been sent to the « Ministère de l’Enseignement Supérieur et de la Recherche » (n°DC-2014-2086).

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Patients were informed about the storage and processing of their specimens and were allowed to withhold their consent for their use.

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Handling Editor: Zhongjie Shi.

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Debernardi, A., Jarassier, W., Soret, C. et al. Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses. Arch Virol 166, 3421–3425 (2021). https://doi.org/10.1007/s00705-021-05259-9

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  • DOI: https://doi.org/10.1007/s00705-021-05259-9

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