Wheat dwarf virus (WDV) has caused an epidemic in many cereal production areas of Turkey in recent years. There is no current literature data on this important virus in the country. This study presents the extensive molecular characterization of WDV isolates causing serious problems in the Central Anatolia Region between 2019 and 2020. Of the 51 locations, 44 locations (25 barley + 19 wheat) were found to be infected with WDV and 7 locations (1 barley + 6 wheat) were WDV-negative by PCR (Polymerase chain reaction). To differentiate the wheat- and barley-specific (WDV-W and WDV-B) forms of WDV, PCR tests were also performed. Eleven locations were identified as WDV-W, and 33 locations were identified as WDV-B. Although two Turkish isolates in WDV-B specific form have been reported in previous studies, isolates belonging to WDV-W specific form were identified for the first time by this study. Partial sequences of randomly selected 16 isolates (8 WDV-W and 8 WDV-B) were realized and deposited in GenBank. The phylogenetic tree was generated by the neighbor-joining method, and 16 isolates were clustered as follows: two isolates in WDV-A, six isolates in WDV-B, and eight isolates in WDV-E. To our knowledge, this study is the first report of B and E strains of WDV in Turkey. This is the first comprehensive study presenting both specific form prevalence and the strains of WDV in Turkey.

近年来，小麦矮化病毒（WDV）在土耳其许多谷物产区引起流行。该国目前没有关于这种重要病毒的文献数据。本研究展示了 2019 年至 2020 年期间在安纳托利亚中部地区造成严重问题的 WDV 分离株的广泛分子特征。在 51 个地点中，发现 44 个地点（25 个大麦 + 19 个小麦）感染了 WDV，7 个地点（1 个大麦） + 6 个小麦）通过 PCR（聚合酶链反应）呈 WDV 阴性。为了区分小麦和大麦特异性（WDV-W 和 WDV-B）形式的 WDV，还进行了 PCR 测试。11 个位置被确定为 WDV-W，33 个位置被确定为 WDV-B。尽管之前的研究报告了两种 WDV-B 特定形式的土耳其分离株，本研究首次鉴定了属于 WDV-W 特定形式的分离株。实现了随机选择的 16 个分离株（8 个 WDV-W 和 8 个 WDV-B）的部分序列并存放在 GenBank 中。系统发育树是通过邻接法生成的，16 个分离株的聚类如下：WDV-A 中的 2 个分离物，WDV-B 中的 6 个分离物，WDV-E 中的 8 个分离物。据我们所知，这项研究是土耳其首次报道 WDV B 和 E 株。这是第一项综合研究，介绍了土耳其 WDV 的特定形式流行情况和毒株。WDV-A 中有 2 个分离物，WDV-B 中有 6 个分离物，WDV-E 中有 8 个分离物。据我们所知，这项研究是土耳其首次报道 WDV B 和 E 株。这是第一项综合研究，介绍了土耳其 WDV 的特定形式流行情况和毒株。WDV-A 中有 2 个分离物，WDV-B 中有 6 个分离物，WDV-E 中有 8 个分离物。据我们所知，这项研究是土耳其首次报道 WDV B 和 E 株。这是第一项综合研究，介绍了土耳其 WDV 的特定形式流行情况和毒株。