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Phylogenetic diversity of barley- and wheat-specific forms of Wheat dwarf virus in Turkey

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Abstract

Wheat dwarf virus (WDV) has caused an epidemic in many cereal production areas of Turkey in recent years. There is no current literature data on this important virus in the country. This study presents the extensive molecular characterization of WDV isolates causing serious problems in the Central Anatolia Region between 2019 and 2020. Of the 51 locations, 44 locations (25 barley + 19 wheat) were found to be infected with WDV and 7 locations (1 barley + 6 wheat) were WDV-negative by PCR (Polymerase chain reaction). To differentiate the wheat- and barley-specific (WDV-W and WDV-B) forms of WDV, PCR tests were also performed. Eleven locations were identified as WDV-W, and 33 locations were identified as WDV-B. Although two Turkish isolates in WDV-B specific form have been reported in previous studies, isolates belonging to WDV-W specific form were identified for the first time by this study. Partial sequences of randomly selected 16 isolates (8 WDV-W and 8 WDV-B) were realized and deposited in GenBank. The phylogenetic tree was generated by the neighbor-joining method, and 16 isolates were clustered as follows: two isolates in WDV-A, six isolates in WDV-B, and eight isolates in WDV-E. To our knowledge, this study is the first report of B and E strains of WDV in Turkey. This is the first comprehensive study presenting both specific form prevalence and the strains of WDV in Turkey.

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Department of Virology, Directorate of Plant Protection Central Research Institute.

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Acknowledgements

This work was supported by Republic of Turkey Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies, Directorate of Plant Protection Central Research Institute.

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Authors and Affiliations

  1. Directorate of Plant Protection Central Research Institute, Gayret Mh. Fatih Sultan Mehmet Bul., Yenimahalle, 06172, Ankara, Turkey

    Ali Ferhan Morca, Sevgi Coskan & Birol Akbas

Authors
  1. Ali Ferhan Morca
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  2. Sevgi Coskan
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  3. Birol Akbas
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All authors contributed to the study conception and design. All authors read and approved the final manuscript.

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Correspondence to Ali Ferhan Morca.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Communicated by K. Posta.

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Morca, A.F., Coskan, S. & Akbas, B. Phylogenetic diversity of barley- and wheat-specific forms of Wheat dwarf virus in Turkey. CEREAL RESEARCH COMMUNICATIONS 50, 1029–1036 (2022). https://doi.org/10.1007/s42976-021-00219-0

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