A double-stranded RNA (dsRNA) of approximately 16 kbp was isolated from symptomless common buckwheat (Fagopyrum esculentum) plants. The size of the dsRNA suggested that it was the replicative form of an endornavirus. The dsRNA was sequenced, and it consisted of 15,677 nt, containing a single open reading frame that potentially encoded a polyprotein of 5190 aa. The polyprotein contained conserved domains for a viral methyltransferase, viral RNA helicase 1, MSCRAMM family adhesion SdrC, UDP-glycosyltransferase, and viral RNA-dependent RNA polymerase 2. A site-specific nick in the plus strand was detected near the 5' end of the dsRNA. BLASTp analysis showed that the polyprotein shared the highest identity with the polyprotein of winged bean endornavirus 1. Results of phylogenetic analysis supported placing this novel virus from common buckwheat, which was provisionally named "Fagopyrum esculentum endornavirus 1", in the genus Alphaendornavirus of the family Endornaviridae.

从无症状的普通荞麦（Fagopyrum esculentum） 植物。dsRNA 的大小表明它是内皮病毒的复制形式。对 dsRNA 进行了测序，它由 15,677 nt 组成，包含一个可能编码 5190 aa 多蛋白的开放阅读框。多蛋白包含病毒甲基转移酶、病毒 RNA 解旋酶 1、MSCRAMM 家族粘附 SdrC、UDP-糖基转移酶和病毒 RNA 依赖性 RNA 聚合酶 2 的保守结构域。在 5' 末端附近检测到正链中的位点特异性切口dsRNA。BLASTp 分析表明，该多蛋白与翅豆内皮病毒 1 的多蛋白具有最高的同一性。系统发育分析的结果支持将这种新病毒置于普通荞麦中，暂命名为“Fagopyrum esculentum endornavirus 1”，属于内分泌病毒科。