This study aimed to determine the antifungal and antibiofilm activities of Agelas dispar on biofilm-producing Candida species. The methanolic extract of A. dispar was obtained and the fraction Ag2 showed inhibitory activity for all 13 Candida strains tested, in concentrations ranging from 2.5 to 0.15625 mg/mL. Antifungal activity of fungicidal nature was seen between 5.0 and 0.3125 mg/mL of extract against the strains. All the strains were classified as biofilm producers. The methanolic extract Ag2 was tested at concentrations of 2.5 and 1.25 mg/mL for antibiofilm activity against the biofilm formation and maturation in all the strains of the genus Candida. Treated and untreated biofilm samples were selected for visualization using scanning electron microscopy (SEM). SEM allowed the visualization of the quantitative decrease in the microbial community, alterations of structural morphology, and destruction of both the formation and maturation of biofilms, at the cellular level. The mechanism of action of this fraction is suggested to be at the plasma membrane and/or cell wall alteration level. Therefore, the use of the methanolic extract of A. dispar may be a promising antifungal and antibiofilm therapeutic strategy against different species of the genus Candida.

本研究旨在确定Agelas dispar对产生生物膜的念珠菌物种的抗真菌和抗生物膜活性。获得了A. dispar的甲醇提取物，组分 Ag2 对所有 13种测试的念珠菌菌株均显示出抑制活性，浓度范围为 2.5 至 0.15625 mg/mL。在 5.0 和 0.3125 mg/mL 提取物之间观察到针对菌株的杀真菌性质的抗真菌活性。所有菌株都被归类为生物膜生产者。在 2.5 和 1.25 mg/mL 的浓度下测试了甲醇提取物 Ag2 对念珠菌属所有菌株中生物膜形成和成熟的抗生物膜活性. 使用扫描电子显微镜 (SEM) 选择处理和未处理的生物膜样品进行可视化。SEM 允许在细胞水平上可视化微生物群落的数量减少、结构形态的改变以及生物膜的形成和成熟的破坏。该部分的作用机制被认为是在质膜和/或细胞壁改变水平。因此，使用A. dispar的甲醇提取物可能是一种有前途的针对念珠菌属不同物种的抗真菌和抗生物膜治疗策略。