Abstract

Among the wide range of biologically active substances secreted by endophytic bacteria, lipopeptides are of considerable interest. The presence of lipopeptide synthetase genes responsible for their synthesis in bacteria of the endophytic strain Bacillus subtilis 26D was analyzed. A lipopeptide-rich fraction was isolated from the culture medium of B. subtilis 26D. Analysis of the lipopeptide composition via thin-layer chromatography (TLC) revealed the presence of surfactin in the B. subtilis 26D strain, which is confirmed by the presence of the surfactin-synthetase gene in the genome of this bacterial strain. High-performance chromatography (HPLC) with mass spectrometric analysis showed the presence of two varieties of surfactin (C13 and C15).

中文翻译：

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枯草芽孢杆菌26D脂肽的分离鉴定

摘要

在内生细菌分泌的多种生物活性物质中，脂肽具有相当大的意义。分析了在内生菌株枯草芽孢杆菌26D 的细菌中负责合成脂肽合成酶基因的存在。从枯草芽孢杆菌26D 的培养基中分离出富含脂肽的部分。通过薄层色谱 (TLC) 对脂肽组成的分析揭示了枯草芽孢杆菌26D 菌株中存在表面活性素，这一点通过该菌株基因组中表面活性素合成酶基因的存在得到证实。带质谱分析的高效色谱 (HPLC) 显示存在两种表面活性素（C13 和 C15）。