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Isolation and Identification of Lipopeptides of Bacillus subtilis 26D

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Abstract

Among the wide range of biologically active substances secreted by endophytic bacteria, lipopeptides are of considerable interest. The presence of lipopeptide synthetase genes responsible for their synthesis in bacteria of the endophytic strain Bacillus subtilis 26D was analyzed. A lipopeptide-rich fraction was isolated from the culture medium of B. subtilis 26D. Analysis of the lipopeptide composition via thin-layer chromatography (TLC) revealed the presence of surfactin in the B. subtilis 26D strain, which is confirmed by the presence of the surfactin-synthetase gene in the genome of this bacterial strain. High-performance chromatography (HPLC) with mass spectrometric analysis showed the presence of two varieties of surfactin (C13 and C15).

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ACKNOWLEDGMENTS

The study was carried out on the equipment of the Agidel Collective Use Center and the unique scientific installation KODINK. The LPs were identified at the Collective Use Center of the Federal Research Center “Kazan Scientific Center of the Russian Academy of Sciences”.

Funding

The study was carried out with the financial support of Russian Foundation for Basic Research (project no. ofi_m 17-29-08014) and partly of state task topic nos. AAAA-A21-121011990120-7 and АААА-А16-116020350033-8.

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  1. Institute of Biochemistry and Genetics, Ufa Federal Research Center, Russian Academy of Sciences, Ufa, Russia

    E. A. Cherepanova, I. V. Galyautdinov, G. F. Burkhanova & I. V. Maksimov

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  1. E. A. Cherepanova
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  2. I. V. Galyautdinov
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Correspondence to I. V. Maksimov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Cherepanova, E.A., Galyautdinov, I.V., Burkhanova, G.F. et al. Isolation and Identification of Lipopeptides of Bacillus subtilis 26D. Appl Biochem Microbiol 57, 636–642 (2021). https://doi.org/10.1134/S0003683821050033

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