Abstract

This paper studies the characteristics of the kinetic interaction of lytic polysaccharide monooxygenases (LPMOs) from Thielavia terrestris (TtLPMO), Penicillium verruculosum (PvLPMO), and Trichoderma reesei (TrLPMO) with purified cellobiohydrolases (CBH I and CBH II) and endoglucanase II (EG II) of P. verruculosum during the enzymatic destruction of microcrystalline (Avicel) and amorphous cellulose. TtLPMO belongs to the C1-type of LPMOs, while PvLPMO and TrLPMO are of the mixed C1/C4-type, according to the generally accepted classification of this class of oxidases. Under the action of any of the three LPMO together with CBH II or EG II on Avicel or amorphous cellulose, respectively, the enzymes displayed synergism, which was manifested in an increase in the yield of reducing sugars (RS). The synergism was expressed to the greatest extent in the initial reaction period. The mixtures of PvLPMO and TrLPMO with CBH I also demonstrated synergism on Avicel as a substrate, while TtLPMO, on the contrary, had an inhibitory effect on RS formation from cellulose with CBH I. The observed synergism and antagonism between LPMOs and CBH II/CBH I can be explained in terms of a simple kinetic scheme, in which the first enzyme forms additional ends of polysaccharide molecules that serve as a substrate or inhibitor for the second enzyme.

中文翻译：

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纤维素的酶促破坏：裂解多糖单加氧酶和单个纤维素酶的动力学相互作用特征

摘要

本文研究了土生梭孢壳(TtLPMO)、疣状青霉(PvLPMO) 和里氏木霉(TrLPMO)的裂解多糖单加氧酶 ( LPMO) 与纯化的纤维二糖水解酶 (CBH I 和 CBH II) 和内切葡聚糖酶 II (EG)的动力学相互作用特征。II) P. verruculosum在微晶（Avicel）和无定形纤维素的酶促破坏过程中。根据对此类氧化酶的普遍接受的分类，TtLPMO 属于 LPMO 的 C1 型，而 PvLPMO 和 TrLPMO 属于混合 C1/C4 型。在三种LPMO中的任何一种与CBH II或EG II一起分别对Avicel或无定形纤维素的作用下，酶表现出协同作用，这表现为还原糖（RS）产率的增加。协同作用在反应初期得到最大程度的体现。PvLPMO 和 TrLPMO 与 CBH I 的混合物在 Avicel 作为底物上也表现出协同作用，而 TtLPMO 相反，对纤维素与 CBH I 的 RS 形成具有抑制作用。