The complete genome of a novel mycovirus, Albatrellopsis flettii mitovirus 1 (AfMV1), hosted by the basidiomycetous ectomycorrhizal fungus Albatrellopsis flettii (Morse ex Pouzar) Audet, was sequenced and analyzed. The full-length cDNA sequence, obtained from a dsRNA replication intermediate of the AfMV1 genome, is 3037 bp in length with a predicted G+C content of 40.66%. Sequence analysis revealed that a single large open reading frame (ORF) is present on the positive strand when the mold mitochondrial genetic code is applied. The single ORF encodes a putative RNA-dependent RNA polymerase of 859 amino acids with a predicted molecular weight of 97.05 kDa that shares the closest similarity with the corresponding protein of Entomophthora muscae mitovirus 7, with 43.38% sequence identity. Phylogenetic analysis showed that AfMV1 could be classified as a new member of the genus Mitovirus within the family Mitoviridae. This is the first report of the complete genome sequence of a new mitovirus, AfMV1, isolated from the basidiomycetous ectomycorrhizal fungus A. flettii.

中文翻译：

---

由外生菌根真菌 Albatrellopsis flettii 宿主的新有丝分裂病毒的分子特征。

对由担子菌外生菌根真菌 Albatrellopsis flettii (Morse ex Pouzar) Audet 宿主的新型真菌病毒 Albatrellopsis flettii mitovirus 1 (AfMV1) 的完整基因组进行了测序和分析。从 AfMV1 基因组的 dsRNA 复制中间体获得的全长 cDNA 序列长度为 3037 bp，预测的 G+C 含量为 40.66%。序列分析表明，当应用霉菌线粒体遗传密码时，正链上存在一个大的开放阅读框 (ORF)。单个 ORF 编码一个推定的 859 个氨基酸的 RNA 依赖性 RNA 聚合酶，预测的分子量为 97.05 kDa，与 Entomophthora muscae mitovirus 7 的相应蛋白质具有最相似的相似性，具有 43.38% 的序列同一性。系统发育分析表明，AfMV1 可被归类为丝裂病毒科中丝裂病毒属的新成员。这是从担子菌外生菌根真菌 A. flettii 中分离出的一种新的有丝分裂病毒 AfMV1 的完整基因组序列的第一份报告。