With the recent developments in the field of nanotechnology, the biosynthesis of nanoparticles has increased tremendously. Silver nanoparticles (SNPs) are among the most synthesized nanoparticles and this extensive synthesis can elevate the amounts of SNPs in the environment, which, consequently, pose a serious threat to the ecosystem and can bring unwanted environmental effects. As plants are an important part of ecosystem, investigation of toxic effects of SNPs on plants is particularly interesting. This study evaluates the potential risk of SNPs interaction with plants. For this, seeds of Vigna radiata L. were screened in presence of SNPs (20 mgL⁻¹) using the germination, growth, and biochemical parameters as a phototoxicity criterion. The 19.57 nm average-sized SNPs were synthesized via the biosynthesis method. These biosynthesized SNPs were then applied on two varieties of V. radiata (Azri and High cross 404) and found to have variety dependent toxic effects on seed germination, growth, and biochemical parameters. Seed germination, root length, shoot length, fresh weight, chlorophyll, carotenoid, sugar content, and total proteins were reduced by 20, 46, 50, 18, 55, 62, 82, and 67%, respectively, in High cross 404, when compared with control (distilled water). The variety Azri was less sensitive than the variety High cross 404. In conclusion, the results demonstrated that SNPs affect seed germination and seedling growth when internalized and accumulated in plants, revealing that SNPs were responsible for the side effects. More in-depth research is required, in the form of different concentrations of SNPs or different plant species, to draw a logical conclusion and develop legislation about the safe use of biosynthesized SNPs.

随着纳米技术领域的最新发展，纳米粒子的生物合成已大大增加。银纳米粒子 (SNP) 是合成最多的纳米粒子之一，这种广泛的合成可以提高环境中 SNP 的数量，从而对生态系统构成严重威胁，并可能带来不必要的环境影响。由于植物是生态系统的重要组成部分，因此研究 SNP 对植物的毒性作用特别有趣。本研究评估了 SNP 与植物相互作用的潜在风险。为此，在存在 SNP（20 mgL ^-1) 使用发芽、生长和生化参数作为光毒性标准。通过生物合成方法合成了平均大小为 19.57 nm 的 SNP。然后将这些生物合成的 SNP 应用于两个品种的V. radiata（Azri 和 High cross 404）并发现对种子萌发、生长和生化参数具有多种依赖性毒性作用。在高杂交 404 中，种子发芽率、根长、芽长、鲜重、叶绿素、类胡萝卜素、糖含量和总蛋白分别降低了 20%、46%、50%、18%、55%、62%、82% 和 67%，与对照（蒸馏水）相比。品种 Azri 的敏感性低于品种 High cross 404。综上所述，结果表明 SNP 在植物内化和积累时会影响种子萌发和幼苗生长，表明 SNP 是造成副作用的原因。需要以不同浓度的 SNP 或不同植物物种的形式进行更深入的研究，以得出合乎逻辑的结论并制定有关安全使用生物合成 SNP 的立法。