Olea europaea geminivirus (OEGV) from olive accessions in Italy was characterized recently. OEGV was also detected during routine high-throughput sequencing screening of olive (cv. Leccino) material, and its complete bipartite genome segments were sequenced and shown to be 100% identical to those of the isolate from Italy. Using two pairs of newly designed primers targeting the AV1 and BV1 genes, OEGV was detected in randomly sampled olive trees from the U.S. Department of Agriculture National Clonal Germplasm Repository (USDA-NCGR) (21.4% or 6/28), commercial and residential settings in California (47.6% or 10/21), and an orchard in Texas (60% or 30/50). The cuttings for the USDA-NCGR-positive trees originated from the former Serbia and Montenegro, Spain, Italy, and Greece. Comparative analysis of the directly sequenced gene fragments from randomly selected samples showed that OEGV isolates from the different sources were 100% identical to each other. The results indicate that OEGV spread was likely facilitated by inadvertent movement of contaminated olive germplasm.

最近对来自意大利橄榄种质的油橄榄双子病毒 (OEGV) 进行了表征。在橄榄（cv. Leccino）材料的常规高通量测序筛选过程中也检测到了 OEGV，对其完整的二分基因组片段进行了测序，并显示与来自意大利的分离株 100% 相同。使用两对新设计的针对 AV1 和 BV1 基因的引物，在美国农业部国家克隆种质资源库 (USDA-NCGR)（21.4% 或 6/28）、商业和住宅环境中随机取样的橄榄树中检测到 OEGV在加利福尼亚州（47.6% 或 10/21）和德克萨斯州的一个果园（60% 或 30/50）。USDA-NCGR 阳性树木的插条起源于前塞尔维亚和黑山、西班牙、意大利和希腊。从随机选择的样本中直接测序的基因片段的比较分析表明，来自不同来源的 OEGV 分离物彼此 100% 相同。结果表明，受污染的橄榄种质的无意移动可能促进了 OEGV 的传播。