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Olea europaea geminivirus is present in a germplasm repository and in California and Texas olive (Olea europaea L.) groves

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Abstract

Olea europaea geminivirus (OEGV) from olive accessions in Italy was characterized recently. OEGV was also detected during routine high-throughput sequencing screening of olive (cv. Leccino) material, and its complete bipartite genome segments were sequenced and shown to be 100% identical to those of the isolate from Italy. Using two pairs of newly designed primers targeting the AV1 and BV1 genes, OEGV was detected in randomly sampled olive trees from the U.S. Department of Agriculture National Clonal Germplasm Repository (USDA-NCGR) (21.4% or 6/28), commercial and residential settings in California (47.6% or 10/21), and an orchard in Texas (60% or 30/50). The cuttings for the USDA-NCGR-positive trees originated from the former Serbia and Montenegro, Spain, Italy, and Greece. Comparative analysis of the directly sequenced gene fragments from randomly selected samples showed that OEGV isolates from the different sources were 100% identical to each other. The results indicate that OEGV spread was likely facilitated by inadvertent movement of contaminated olive germplasm.

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References

  1. Koudounas K, Thomopoulou M, Angeli E et al (2020) Virus-induced gene silencing in olive tree (Oleaceae). In: Courdavault V, Besseau S (eds) Virus-induced gene silencing in plants: methods and protocols. Springer US, New York, pp 165–182

    Chapter  Google Scholar 

  2. Miazzi MM, di Rienzo V, Mascio I et al (2020) Re.Ger.O.P.: an integrated project for the recovery of ancient and rare olive germplasm. Front Plant Sci 11:73

    Article  Google Scholar 

  3. Martelli GP (2013) A brief outline of infectious diseases of olive. /paper/A-Brief-Outline-of-Infectious-Diseases-of-Olive-Martelli/234ee0f46d7b4b7ae285aa4a024b3e27efc1af25. Accessed 16 Jun 2020

  4. Savino V, Gallitelli D, Barba M (1983) Olive latent ringspot virus, a newly recognised virus infecting olive in Italy. Ann Appl Biol 103:243–249

    Article  CAS  Google Scholar 

  5. Marte M, Gadani F, Savino V et al (1986) Strawberry latent ringspot virus associated with a new disease of olive in Central Italy. Plant Dis 70:171–172

    Article  Google Scholar 

  6. Gallitelli D, Savino V (1985) Olive latent virus-1, an isometric virus with a single RNA species isolated from olive in Apulia, Southern Italy. Ann Appl Biol 106:295–303

    Article  CAS  Google Scholar 

  7. Martelli GP, Grieco F (1997) Oleavirus, a new genus in the family Bromoviridae. Adv Virol 142:1933–1936

    CAS  Google Scholar 

  8. Alabdullah A, Minafra A, Elbeaino T et al (2010) Complete nucleotide sequence and genome organization of olive latent virus 3, a new putative member of the family Tymoviridae. Virus Res 152(1–2):10–18

    Article  CAS  Google Scholar 

  9. Sabanadzovic S, Abou-Ghanem N, La Notte P et al (1999) Partial molecular characterization and RT-PCR detection of a putative closterovirus associated with olive leaf yellowing. J Plant Pathol 81:37–45

    CAS  Google Scholar 

  10. Cardoso JMS, Félix MR, Clara MIE et al (2005) The complete genome sequence of a new necrovirus isolated from Olea europaea L. Adv Virol 150:815–823

    CAS  Google Scholar 

  11. Chiumenti M, Greco C, Antelmi I et al (2019) Molecular characterisation of a novel gemycircularvirus associated with olive trees in Italy. Virus Res 263:169–172

    Article  CAS  Google Scholar 

  12. Chiumenti M, Greco C, De Stradis A et al (2021) Olea europaea geminivirus: a novel bipartite geminivirid infecting olive trees. Viruses 13:481. https://doi.org/10.3390/v13030481

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Al Rwahnih M, Rowhani A, Westrick N et al (2018) Discovery of viruses and virus-like pathogens in pistachio using high-throughput sequencing. Plant Dis 102:1419–1425

    Article  CAS  Google Scholar 

  14. Tatusova TA, Madden TL (1999) BLAST 2 sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174:247–250

    Article  CAS  Google Scholar 

  15. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  16. Hall T, Biosciences I, Carlsbad C (2011) BioEdit: an important software for molecular biology. GERF Bull Biosci 2:60–61

    Google Scholar 

  17. Rojas MR, Gilbertson RL, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347

    Article  CAS  Google Scholar 

  18. Bull SE, Briddon RW, Markham PG (2003) Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA β complexes. Mol Biotechnol 23:83–86

    Article  CAS  Google Scholar 

  19. Briddon RW, Bull SE, Mansoor S et al (2002) Universal primers for the PCR-mediated amplification of DNA β. Mol Biotechnol 20:315–318

    Article  CAS  Google Scholar 

  20. Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 9:e108277

    Article  Google Scholar 

  21. Martin DP, Murrell B, Golden M et al (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 1(1):vev003

    Article  Google Scholar 

  22. Diaz-Lara A, Golino D, Al Rwahnih M (2018) Genomic characterization of grapevine virus J, a novel virus identified in grapevine. Arch Virol 163:1965–1967

    Article  CAS  Google Scholar 

  23. Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035

    Article  CAS  Google Scholar 

  24. Kumar S, Stecher G, Li M et al (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    Article  CAS  Google Scholar 

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Acknowledgements

We are grateful to Jenny Smith (USDA National Clonal Germplasm Repository) for help with sample collection, and Cecilia Villegas (Texas A&M AgriLife Research and Extension Center, Weslaco, TX) for technical assistance.

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Authors and Affiliations

  1. Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX, 78596, USA

    Olufemi J. Alabi

  2. Department of Plant Pathology, University of California-Davis, Davis, CA, 95616, USA

    Alfredo Diaz-Lara, Teresa M. Erickson & Maher Al Rwahnih

  3. School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, 76130, Querétaro, Mexico

    Alfredo Diaz-Lara

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  1. Olufemi J. Alabi
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  2. Alfredo Diaz-Lara
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  4. Maher Al Rwahnih
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Corresponding author

Correspondence to Maher Al Rwahnih.

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All the authors declare no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Elvira Fiallo-Olivé.

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The nucleotide sequences reported in this work were deposited in the GenBank database under accession numbers MW560446 to MW560455 (complete DNA-A); MW814511 (complete DNA-B); MW939498 to MW939536, MZ501347 to MZ501351 (partial AV1); MW939537 to MW939575, and MZ501352 to MZ501356 (partial BV1).

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Alabi, O.J., Diaz-Lara, A., Erickson, T.M. et al. Olea europaea geminivirus is present in a germplasm repository and in California and Texas olive (Olea europaea L.) groves. Arch Virol 166, 3399–3404 (2021). https://doi.org/10.1007/s00705-021-05218-4

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  • DOI: https://doi.org/10.1007/s00705-021-05218-4

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