Aedes aegypti is an important vector of dengue fever, dengue hemorrhagic fever and yellow fever, chikungunya, and Zika virus. The objective was to evaluate the resistance of A. aegypti exposed to insecticides with different action modes (deltamethrin, imidacloprid, and fipronil) under intense selection pressure for 10 generations in laboratory. Bioassays were conducted according to World Health Organization. Biochemical assay performed after selection with deltamethrin (Delta-SEL), fipronil (Fipro-SEL), and imidacloprid (Imida-SEL) from G₁ to G₁₀ was used for the assessment of detoxification enzymes (esterase (EST), acetylcholinesterase (AChE), glutathione S-transferases (GST), and acid and alkaline phosphatases (ACP and ALP)). The Fipro-SEL (G₁₀) had high resistance (77-fold), whereas Delta-SEL and Imida-SEL populations presented very high resistance with 118 and 372-fold, respectively, in comparison with unselected (UNSEL). The levels of EST, AChE, GST, ACP, and ALP enzymes amplified on application from G₁ to G₁₀. The enzymes contributing in resistance development of insecticides were as follows: GST (20.7 µmol/min/mg of protein) in Delta-SEL (G₁₀), while AChE 9.71 µmol/min/mg of protein in Imida-SEL (G₁₀) and the peak ACP and ALP enzyme activities 13.32 and 12.93 µmol/min/mg of protein, respectively, in Fipro-SEL (G₁₀). The results showed that detoxification enzymes trigger insecticide resistance in A. aegypti and their suppression may aid in the resistance breakage.

埃及伊蚊是登革热、登革出血热和黄热病、基孔肯雅热和寨卡病毒的重要媒介。目的是在实验室中评估 10 代在强烈选择压力下暴露于具有不同作用模式（溴氰菊酯、吡虫啉和氟虫腈）的杀虫剂的埃及伊蚊的抗性。根据世界卫生组织进行生物测定。_{使用溴氰菊酯 (Delta-SEL)、氟虫腈 (Fipro-SEL) 和吡虫啉 (Imida-SEL) 从 G 1}到 G ₁₀进行选择后进行的生化测定用于评估解毒酶 (酯酶 (EST)、乙酰胆碱酯酶 (AChE) )、谷胱甘肽 S-转移酶 (GST) 以及酸性和碱性磷酸酶 (ACP 和 ALP))。Fipro-SEL (G₁₀ ) 具有高抗性 (77 倍)，而与未选择 (UNSEL) 相比，Delta-SEL 和 Imida-SEL 种群分别表现出非常高的抗性，分别为 118 倍和 372 倍。EST、AChE、GST、ACP 和 ALP 酶的水平在从 G ₁到 G ₁₀的应用中扩增。影响杀虫剂抗性发展的酶如下：Delta-SEL (G 10 ) 中的 GST (20.7 µmol/min/mg 蛋白质) _，而 Imida-SEL (G ₁₀ )中的 AChE 9.71 µmol/min/mg 蛋白质Fipro-SEL (G ₁₀ )中的峰值 ACP 和 ALP 酶活性分别为 13.32 和 12.93 µmol/min/mg 蛋白质。结果表明，解毒酶引发了埃及伊蚊的杀虫剂抗性。并且它们的抑制可能有助于抵抗破坏。